Antinociceptive and anti-inflammatory kaempferol glycosides from Sedum dendroideum |
| |
Authors: | Giany O De Melo David do C Malvar Frederico A Vanderlinde Fabio F Rocha Priscila Andrade Pires Elson A Costa Lécia G de Matos Carlos R Kaiser Sônia S Costa |
| |
Institution: | 1. Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil;2. Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, 23850-000 Seropédica, RJ, Brazil;3. Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-131 Goiânia, GO, Brazil;4. Instituto de Química, Universidade Federal do Rio de Janeiro, 21941-909 Rio de Janeiro, RJ, Brazil |
| |
Abstract: | Aim of the studyTo identify the compounds responsible for the antinociceptive and anti-inflammatory effects previously described for Sedum dendroideum, through bioassay-guided fractionation procedures.Materials and methodsAntinociceptive activity was evaluated through mouse acetic acid-induced writhing model. The anti-inflammatory activity was assessed through croton oil-induced mouse ear oedema and carrageenan-induced peritonitis.ResultsThe Sedum dendroideum juice afforded seven known flavonoids identified with basis on NMR data. The oral administration of the major kaempferol glycosides kaempferitrin 1] (17.29 μmol/kg), kaempferol 3-O-β-glucopyranoside-7-O-α-rhamnopyranoside 2] (16.82 μmol/kg), kaempferol 3-O-neohesperidoside-7-O-α-rhamnopyranoside 3] (13.50 μmol/kg) or α-rhamnoisorobin 5] (23.13 μmol/kg) inhibited by 47.3%, 25.7%, 60.2% and 58.0%, respectively, the acetic acid-induced nociception (indomethacin: 27.95 μmol/kg, p.o.; 68.9%). Flavonoids 1, 2, 3 or 5, at the same doses, reduced by 39.5%, 46.5%, 35.6% and 33.3%, respectively, the croton oil-induced oedema (dexamethasone: 5.09 μmol/kg, s.c.; 83.7%) and impaired leukocyte migration by 42.9%, 46.3%, 50.4% and 49.6%, respectively (dexamethasone: 5.09 μmol/kg, s.c.; 66.1%).ConclusionsOur findings show that the major kaempferol glycosides may account for the renowned medicinal use of Sedum dendroideum against pain and inflammatory troubles. |
| |
Keywords: | LJ lyophilized Sedum dendroideum crude juice Sd-Ppt precipitate fraction Sd-Sn supernatant fraction FA EtOAc fraction FB n-BuOH fraction CGRP calcitonin gene-related peptide p o per os s c subcutaneous i p intraperitoneal Dexa dexamethasone Indo indomethacin iNOS inductible nitric oxide synthase |
本文献已被 ScienceDirect 等数据库收录! |
|