Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP-induced cytotoxicity |
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Authors: | In Su Kim Sushruta Koppula Pyo-Jam Park Ee Hwa Kim Chan Gil Kim Wahn Soo Choi Kwang Ho Lee Dong-Kug Choi |
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Institution: | 1. Department of Biotechnology, Konkuk University, 322 Danwol-dong, Chungju 380-701, Republic of Korea;2. Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University, Chungbuk, Republic of Korea;3. College of Medicine, Konkuk University, Chungju, Republic of Korea |
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Abstract: | Ethnopharmacological relevanceChrysanthemum morifolium Ramat (Asteraceae) has (CM) long been used in Korean and Chinese traditional herbal medicines with numerous therapeutic applications.Aim of the studyTo evaluate the neuroprotective activities of Chrysanthemum morifolium (CM) extract against 1-methyl-4-phenylpridinium ions (MPP+), Parkinsonian toxin through oxidative stress and impaired energy metabolism, in human SH-SY5Y neuroblastoma cells and the underlying mechanisms.Materials and methodsThe effects of CM against MPP+-induced cytotoxicity and neuronal cell viability, oxidative damage, the expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase (PARP) proteolysis were evaluated by using SH-SY5Y neuroblastoma cells.ResultsCM effectively inhibited the cytotoxicity and improved cell viability. CM also attenuated the elevation of reactive oxygen species (ROS) level, increase in Bax/Bcl-2 ratio, cleavage of caspase-3 and PARP proteolysis.ConclusionThese results demonstrate that CM possesses potent neuroprotective activity and therefore, might be a potential candidate in neurodegenerative diseases such as Parkinson's disease. |
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Keywords: | Chrysanthemum morifolium (CM) MPP+ Parkinson's disease ROS Caspase Mitochondrial dysfunction |
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