In vitro and in vivo antimicrobial activities of seeds of Caesalpinia bonduc (Lin.) Roxb. |
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Authors: | Tasleem Arif TK Mandal Naresh Kumar JD Bhosale Archana Hole GL Sharma MM Padhi GS Lavekar Rajesh Dabur |
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Institution: | 1. Regional Research Institute (Ay), (CCRAS), Nehru Garden, Kothrud, Pune, MS 411038, India;2. Central Council for Research in Ayurveda and Siddha, Opp ‘D’ Block, Janakpuri, New Delhi, India;3. Institute of Genomics and Integrative Biology, DU Campus, Mall Road, Delhi 110007, India |
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Abstract: | Aim of the studyCaesalpinia bonduc (Lin.) Roxb. is a known drug in Ayurveda to treat various diseases specifically tumors, cysts and cystic fibrosis (CF). The aim of this study was to assess in vitro as well as in vivo antimicrobial activity of Caesalpinia bonduc seeds.Materials and methodsThe in vitro antimicrobial activities of seed coat and seed kernel extracts were investigated by microbroth dilution assay. In vivo activities of hydro-alcoholic extracts were investigated in rat models of chronic Pseudomonas aeruginosa pneumonia mimicking that in patients with cystic fibrosis.ResultsVarious extracts of plant seeds exhibited in vitro antimicrobial activities in a range of 22–350 μg/ml. The extracts also showed activity against methicillin resistant (MR) Staphylococcus aureus and ampicillin resistant (AR) Pseudomonas aeruginosa as in the sensitive strains. In rat model of chronic Pseudomonas aeruginosa pneumonia, hydro-alcoholic extracts of Caesalpinia bonduc seed kernel (CBSK) and Caesalpinia bonduc seed coat (CBSC) were injected subcutaneously in the test groups of animals. The control groups were treated with cortisone and saline. Two weeks after challenge with Pseudomonas aeruginosa, the CBSK treated animals showed a significant bacterial clearance from the lungs (P < 0.04) and less severe incidence of lung abscess (P < 0.05).ConclusionResults showed that Caesalpinia bonduc may have the potential to be promising natural medicine, with other forms of treatments, for CF patients with chronic Pseudomonas aeruginosa lung infections. |
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Keywords: | Caesalpinia bonduc Cystic fibrosis Antimicrobial Lung infection |
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