Huo-Luo-Xiao-Ling Dan modulates antigen-directed immune response in adjuvant-induced inflammation |
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Authors: | Rajesh Rajaiah David Y-W Lee Zhongze Ma Arthur Y Fan Lixing Lao Harry HS Fong Brian M Berman Kamal D Moudgil |
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Institution: | 1. Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD (UMB), United States;2. Mailman Research Center, McLean Hospital, Harvard Medical School, Boston, MA, United States;3. Center for Integrative Medicine, Kernan Hospital, UMB, United States;4. Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL, United States |
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Abstract: | Ethnopharmacological relevanceHLXL is a traditional Chinese medicine that has long been used in folk medicine for the treatment of chronic inflammatory diseases. However, the precise immunological mechanisms by which HLXL mediates its anti-inflammatory activity are not fully defined.Aim of the studyTo determine the effects of HLXL on antigen-specific immune parameters in adjuvant-induced inflammation model in the Lewis rat.Materials and methodsRats were fed daily with either HLXL (2.3 g/kg) or vehicle (water) beginning 3 days before subcutaneous injection of heat-killed Mycobacterium tuberculosis H37Ra (Mtb), and then continued for another 6 days. After 9 days of Mtb injection, the draining lymph node cells were tested for T cell proliferative and cytokine responses against mycobacterial heat-shock protein 65 (Bhsp65). Moreover, sera were tested for anti-Bhsp65 antibodies and nitric oxide (NO).ResultsHLXL-treated rats showed reduced T cell proliferative response to Bhsp65 compared to control rats. Furthermore, HLXL suppressed IL-17 response but enhanced IL-10 response without much effect on IFN-γ. HLXL treatment also reduced the levels of anti-Bhsp65 antibodies but not that of NO.ConclusionsHLXL feeding modulated both the cellular and the humoral immune response to Bhsp65 favoring an anti-inflammatory milieu for the suppression of adjuvant-induced inflammation. |
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Keywords: | Huo-Luo-Xiao-Ling Dan Immune modulation Cytokines Antibodies T cells Inflammation |
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