表达MT1-MMP的乳腺癌细胞株对蒽环类药物敏感性研究

目的观察表达MT1-MMP的乳腺癌细胞株对蒽环类化疗药物敏感性的影响。方法用脂质体Lipofectamine 2000将pcDNA3.1-MT1-MMP真核表达载体转染乳腺癌细胞株MDA-MB-453,然后用含G418的培养基筛选获得过表达MT1-MMP的乳腺癌细胞株。蛋白印迹（Western blotting）技术检测MDA-MB-453细胞内MT1-MMP的表达情况。四唑蓝（MTT）法检测各组细胞对化疗药物的敏感性。结果 Western blotting实验证实,重组载体pcDNA3.1-MT1-MMP真核表达载体成功转入乳腺癌细胞内,并稳定过表达MT1-MMP。MTT法检测发现,阿霉素（ADH）、表阿霉素（EPI）、吡喃阿霉素（THP）转染组细胞的抑制率分别为41.38±1.34%、37.69±1.93%和57.96±3.19%,均明显低于未转染组（70.22±2.55%、70.35±1.21%、76.97±2.70%）和空白质粒组（68.94±1.89%、69.43±1.27%、73.06±1.65%,P〈0.05）。结论表达MT1-MMP的乳腺癌细胞株MDA-MB-453对蒽环类化疗药物具有耐药性,MT1-MMP有可能成为一个新的蒽环类化疗药物敏感性预测因子。

Sensitivity of breast cancer cell line expressing MT1-MMP to anthracyclines

Objective To investigate effect of MT1-MMP overexpression on sensitivity of breast cancer cell line MDA-MB-453 to anthracyclines. Methods The pcDNA3. 1-MT1-MMP eukaryotic expression vector was transfected into breast cancer cell line MDA-MB-453 by Lipofectamine 2000 and screened in medium containing G418 to obtain clones overexpressing MT1-MMP. The expression of MT1-MMP protein was detected by Western blotting methods. Sensitivity of breast cancer cells to chemotherapeutic drugs was detected by methyl thiazolyl tetrazolium（ MTT） assay. Results Western blotting confirmed that the vector pcDNA3. 1-MT1-MMP was successfully transfected into breast cancer cell line MDA-MB-453 and stably overexpressed the MT1-MMP protein. In the MTT assay,the inhibition rates of transfected cells by adriamycin（ ADM） ,epirubicin（ EPI） and pirarubicin（ THP） were 41. 38 ± 1. 34% ,37. 69 ± 1. 93% and 57. 96 ± 3. 19% ,respectively. The chemosensitivity of cells in transfected group was lower than that of cells in blank plasmid group（ 68. 94 ± 1. 89% ,69. 43 ± 1. 27% and 73. 06 ± 1. 65% ） and cells in untransfected group （ 70. 22 ± 2. 55%,70. 35 ± 1. 21%,76. 97 ± 2. 70%） （ P 0. 05 ） . Conclusion Breast cancer cell line MDA-MB-453 expressing MT1-MMP has resistance to anthracycline. MT1-MMP may become a new predictor of chemosensitivity to anthracyclines.