内皮细胞促进胰岛的成活与功能

目的：探讨内皮细胞对胰岛细胞活性与功能的支持作用。方法：内皮细胞和胰岛分别被分离纯化，在共
培养中评估胰岛细胞活性与功能；体内试验分为单纯胰岛移植组、胰岛-内皮共移植组、内皮细胞移植组和PBS对照
组，测定血糖和胰岛素水平，免疫组织化学染色及电镜下观察组织细胞形态、结构及细胞标志物鉴定。结果：培养
7 d，超过90%的胰岛细胞显示正常形态。共培养组中的胰岛素释放水平显著高于胰岛培养组(P<0.05)。移植3 d后，各
移植组的血糖和胰岛素水平明显不同(P<0.05)。胰岛-内皮共移植组和单纯移植组间比较，胰岛存活时间差异有统计
学意义(P=0.001)。结论：与内皮细胞在体外共培养能改善分离的大鼠胰岛细胞活性与功能，同内皮细胞共移植能够
有效地延长糖尿病大鼠胰岛移植物存活期。

Endothelial cells promote islet survival and function

Objective: To investigate islet graft survival and function after co-culture and co-transplantation
with vascular endothelial cells (ECs) in diabetic rats.
Methods: We isolated ECs, and assessed the viability of isolated islets in a group of standard culture
and a group of co-culture with ECs. Then we put the diabetic rats in 4 groups: an islet transplantation
group, an islet graft with EC transplantation group, an EC transplantation group, and a PBS control
group. Blood glucose and insulin concentrations were measured daily. Cell morphology and cell
markers were investigated by immunohistochemical staining and electron microscope.
Results: Normal morphology was shown in more than 90% of AO/PI staining positive
islets while co-cultured with ECs for 7 days. Insulin release assays showed a significantly higher
simulation index co-culture except for the first day (P<0.05). There was a significant difference
in concentrations of blood glucose and insulin among the 4 groups after 3 days after the transplantation (P<0.05).
Conclusion: EC-islet co-culture can improve the function and survival of isolated islets in vitro,
and EC-islet co-transplantation can effectively prolong the islet graft survival in diabetic rats.