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| 正交试验法优选玉屏风总苷的提取工艺 | |
| 引用本文： | 高建,李俊,麻兵继,程文明,金涌,吕雄文,闫晨,王建青,邵旭,葛少祥.正交试验法优选玉屏风总苷的提取工艺[J].中国实验方剂学杂志,2007,13(12):15-17. |
| 作者姓名： | 高建 李俊 麻兵继 程文明 金涌 吕雄文 闫晨 王建青 邵旭 葛少祥 |
| 作者单位： | 1. 安徽医科大学第一附属医院药剂科,安徽,合肥,230022;安徽医科大学药学院,安徽,合肥,230032 2. 合肥七星医药科技有限责任公司,安徽,合肥,230088 3. 安徽医科大学药学院,安徽,合肥,230032 4. 中科院昆明植物所,云南,昆明,6502042 |
| 基金项目： | 安徽省自然科学基金资助项目(050431001) |
| 摘 要： | 目的:优选玉屏风散中有效部位-玉屏风总苷(TGYPF)的提取工艺。方法:设计L9(34)正交试验,采用HPLC-ELSD法测定黄芪甲苷的含量作为质控指标,考察乙醇浓度(A)、乙醇用量(B)、提取时间(C)和提取次数(D)4个因素对玉屏风总苷(TGYPF)提取的影响,优化提取工艺。结果:最佳提取条件为:A2B3C3D2,即80%乙醇,8倍量,提取2次,每次3 h。结论:该提取工艺稳定性和重现性良好,简便,快速,可行。 |
| 关 键 词： | 玉屏风总苷 正交试验 提取 黄芪甲苷 |
| 文章编号： | 1005-9903（2007）12-0015-03 |
| 收稿时间： | 2007-04-02 |
| 修稿时间： | 2007年4月2日 |
Study on the Extraction Process for Total Glucosides of Yupingfeng with Orthogonal Design |
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| GAO Jian,LI Jun,MA Bin-ji,CHENG Wen-ming,JIN Yong,LV Xiong-wen,YAN Chen,WANG Jian-qing,SHAO Xu,GE Shao-xiang.Study on the Extraction Process for Total Glucosides of Yupingfeng with Orthogonal Design[J].China Journal of Experimental Traditional Medical Formulae,2007,13(12):15-17. | |
| Authors: | GAO Jian LI Jun MA Bin-ji CHENG Wen-ming JIN Yong LV Xiong-wen YAN Chen WANG Jian-qing SHAO Xu GE Shao-xiang |
| Institution: | 1.Dept of Pharmacy,The First Affiliated Hospital of Anhui Medical University,Anhui Hefei 230022,China;2.School of Pharmacy,Anhui Medical University,Anhui Hefei 230032,China;3.Kunming Institute of Botany,Chinese Academy of Sciences,Yunnan Kunming 6502042,China;4.Hefei Seven Star Medicine and Technology Co.Ltd,Anhui Hefei 230088,China |
| Abstract: | Objective:To study the optimized extraction process of total glucosides of Yu Ping Feng(TGYPF),one of the effective parts of Yupingfeng.Methods:By use of orthogonal experimental design L_9(3~4),four factors that may effect the extraction had been studied,including alcoholic concentration,alcoholic volume,extracting time and times.And the content of astragloside IV had been developed as its quality standard,screening out the best method of extraction conditions.Results:The best extracting technical conditions were as follows: 8 times amount of 80%EtOH,extracting solvent to reflux for 3 hour and 2 times.Conclusion:The optimized process is simple,fast and feasible,with its stability and repeatability are good respectively. |
| Keywords: | total glucosides of Yupingfeng orthogonal test extraction astragloside IV |
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