寿胎丸含药血清对人早孕滋养层细胞增殖、侵袭、迁移能力的影响

  目的：探讨寿胎丸含药血清对人早孕滋养层细胞增殖、侵袭、迁移能力的调节作用。  方法：体外分离培养人早孕滋养层细胞,采用高、中、低剂量寿胎丸含药血清进行干预,以空白血清、地屈孕酮含药血清为对照。采用噻唑蓝(MTT)比色法、流式细胞术(FCM)及Transwell 侵袭移动实验测定滋养层细胞的增殖活性、周期分布及侵袭迁移能力。  结果：与空白组比较,滋养层细胞经寿胎丸含药血清干预后,细胞的增殖活性、侵袭迁移能力明显增强,S期细胞比例增高,G2/M期细胞比例降低(P<0.05)。与寿胎丸低剂量组比较,寿胎丸中、高剂量组滋养层细胞的增殖活性、侵袭迁移能力明显增强,细胞周期分布变化更加明显(P<0.05)。  结论：寿胎丸含药血清可促进人早孕滋养层细胞的增殖活性及侵袭迁移能力,减少细胞凋亡。

Effects of serum containing Shoutai Pill on proliferation,invasion and migration of human first trimester trophoblastic cells

  Objective:To investigate the regulating effect of serum containing Shoutai Pill on proliferation,invasion and migration of human first trimester trophoblastic cells. Methods Human first trimester trophoblastic cells were isolated and cultured in vitro, and then treated with serum containing Shoutai Pill at high-, medium-and low-dose respectively. Blank serum and serum containing dydrogesterone were taken as the control. The proliferation activity, cell cycle distribution, invasion and migration capacity of trophoblastic cells were detected by methyl thiazolyl tetrazolium (MTT) colorimetry,flow cytometry (FCM) and Transwell experiments respectively.  Results: Compared with the control group treated with blank serum,after intervention of serum containing Shoutai Pill on the trophoblastic cells,the activity of cell proliferation and the capacity of invasion and migration were obviously enhanced,the cellular proportion in S period was increased and the cellular proportion in G2/M period was decreased(P<0.05). Compared with low-dose group of Shoutai Pill,the activity of cell proliferation and the capacity of invasion and migration were obviously enhanced in the trophoblastic cells treated with Shoutai Pill at medium-and high-dose,and the change of cell cycle distribution was more obvious(P<0.05).  Conclusion: Serum containing Shoutai Pill can promote the activity of proliferation and the capacity of invasion and migration of human first trimester trophoblastic cells,and reduce cell apoptosis.