| 骨痛灵方对肺癌骨癌痛模型小鼠的疼痛行为学以及脊髓MCP-1和NGF的影响 |
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| 引用本文： | 罗琴琴,王立芳,徐振晔,金贵玉,于璟璐. 骨痛灵方对肺癌骨癌痛模型小鼠的疼痛行为学以及脊髓MCP-1和NGF的影响[J]. 上海中医药杂志, 2017, 51(2): 81-84 |
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| 作者姓名： | 罗琴琴 王立芳 徐振晔 金贵玉 于璟璐 |
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| 作者单位： | 上海中医药大学附属龙华医院肿瘤科,上海,200032,上海中医药大学附属龙华医院肿瘤科,上海,200032,上海中医药大学附属龙华医院肿瘤科,上海,200032,上海中医药大学附属龙华医院肿瘤科,上海,200032,上海中医药大学附属龙华医院肿瘤科,上海,200032 |
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| 基金项目： | 国家自然科学青年基金资助项目(81202670)；上海市中医药事业发展三年行动计划项目(ZY3-CCCX-2-1002) |
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| 摘 要： | 目的：观察骨痛灵方对肺癌骨癌痛模型小鼠脊髓单核细胞趋化因子(MCP-1)和神经生长因子(NGF)表达的影响,探讨骨痛灵方治疗骨癌痛的潜在作用机制。 方法：在C57小鼠左后肢胫骨骨髓腔注射Lewis肺癌细胞建立骨癌痛模型。50只小鼠随机分为假手术组、模型组、中药组、西药组和中西药组,每组10只。中药组小鼠以骨痛灵方煎剂灌胃,西药组予以唑来膦酸腹腔注射,中西药组则两药联合运用。用Von Frey纤维及热痛测试仪分别于造模前,造模后第6(7)天、13(14)、20(21)天观察小鼠机械性缩足反射阈值(PWMT)和热痛觉缩足潜伏期(PWTL)的变化,酶联免疫吸附反应(ELISA)检测脊髓MCP-1和NGF蛋白的表达情况。 结果：3周后,3个治疗组的PWMT和PWTL均明显高于模型组(均P<0.05),中西药组的PWMT比中药组和西药组均增高,差异有统计学意义(均P<0.05)。与模型组比较,中药组、西药组和中西药组的MCP-1蛋白表达均明显降低(均P<0.05)；NGF则均显著升高(均P<0.05)。 结论：骨痛灵方可能通过减少脊髓MCP-1蛋白的表达,抑制中枢敏化,同时促进NGF蛋白的表达,修复中枢敏化中受损的神经元而发挥止痛作用。
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| 关 键 词： | 骨癌痛 肺癌骨转移 骨痛灵方 单核细胞趋化因子(MCP-1) 神经生长因子(NGF) |
| 收稿时间： | 2016-09-15 |
Effects of Gutongling Prescription on the pain behavior in mice model of lung cancer with bone metastasis and MCP-1 and NGF in the spinal cord |
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| LUO Qin-qin,WANG Li-fang,XU Zhen-ye,JIN Gui-yu and YU Jing-lu. Effects of Gutongling Prescription on the pain behavior in mice model of lung cancer with bone metastasis and MCP-1 and NGF in the spinal cord[J]. Shanghai Journal of Traditional Chinese Medicine, 2017, 51(2): 81-84 |
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| Authors: | LUO Qin-qin WANG Li-fang XU Zhen-ye JIN Gui-yu YU Jing-lu |
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| Affiliation: | Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200032,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200032,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200032,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200032 and Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,200032 |
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