对耐胆盐革兰氏阴性菌检验采用MPN法和平板法的比较 |
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引用本文: | 潘雯,陈伟盛. 对耐胆盐革兰氏阴性菌检验采用MPN法和平板法的比较[J]. 中国药品标准, 2017, 18(3): 199-201 |
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作者姓名: | 潘雯 陈伟盛 |
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作者单位: | 广州市药品检验所,广州市药品检验所 |
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摘 要: | 目的:比较MPN法和平板法对耐胆盐革兰氏阴性菌计数是否存在显著性差异。方法:采用单人四种方法计数和多人两种方法计数,通过统计学分析是否存在显著性差异。结果: MPN的离散性较大,预培养5小时较2小时菌体有增殖。结论:检查耐胆盐革兰氏阴性菌应尽量在2小时内完成,MPN法较灵敏,更适合于污染轻的样品。
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关 键 词: | 耐胆盐革兰氏阴性菌 MPN法 平板法 |
收稿时间: | 2017-02-28 |
修稿时间: | 2017-06-05 |
A comparison of the enumeration of bile-tolerant gram-negative bacteria with MPN method and plate method |
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Affiliation: | Guangzhou Institute For Drug Control, and Guangzhou Institute For Drug Control |
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Abstract: | Object: To compare the MPN method and plate method on enumeration of bile-tolerant gram-negative bacteria. Method: The statistical analyses of the enumerations between MPN method and plate method were used on one-researcher and multi-researchers. Result: The measure of dispersion in the enumeration data from MPN method was greater than plate method. And compared with 2h pre-incubation, bacteria proliferation was found more after 5h pre-incubation in MPN method. Conclusion: The MPN method was more sensitive than plate method so that it was applicable to enumerate bile-tolerant gram-negative bacteria from the sample with a little microbial contamination, and the bacteria should be resuscitated for 2h pre-incubation at most. |
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Keywords: | Bile-tolerant gram-negative bacteria MPN method plate method |
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