对耐胆盐革兰氏阴性菌检验采用MPN法和平板法的比较

目的：比较MPN法和平板法对耐胆盐革兰氏阴性菌计数是否存在显著性差异。方法：采用单人四种方法计数和多人两种方法计数,通过统计学分析是否存在显著性差异。结果： MPN的离散性较大,预培养5小时较2小时菌体有增殖。结论：检查耐胆盐革兰氏阴性菌应尽量在2小时内完成,MPN法较灵敏,更适合于污染轻的样品。

A comparison of the enumeration of bile-tolerant gram-negative bacteria with MPN method and plate method

Object: To compare the MPN method and plate method on enumeration of bile-tolerant gram-negative bacteria. Method: The statistical analyses of the enumerations between MPN method and plate method were used on one-researcher and multi-researchers. Result: The measure of dispersion in the enumeration data from MPN method was greater than plate method. And compared with 2h pre-incubation, bacteria proliferation was found more after 5h pre-incubation in MPN method. Conclusion: The MPN method was more sensitive than plate method so that it was applicable to enumerate bile-tolerant gram-negative bacteria from the sample with a little microbial contamination, and the bacteria should be resuscitated for 2h pre-incubation at most.