日本血吸虫分泌蛋白基因Sjsp16的克隆与表达

目的　克隆和表达日本血吸虫新的分泌蛋白Sjsp16的编码基因。　 方法　根据EST测序的结果设计引物 ,从含有Sjsp16基因的cDNA克隆中扩增得到该编码基因 ,亚克隆入原核表达载体 pET2 8中表达 ,然后用生物信息学的方法对蛋白结构功能进行预测。　结果　成功克隆和表达了日本血吸虫分泌蛋白基因Sjsp16,生物信息学分析提示该基因编码蛋白的N端带有一个信号肽序列 ,是一个分泌蛋白 ,含有一个ML功能结构域 ,具有 4种潜在的功能作用位点 ,即 1个N 糖基化位点、3个蛋白激酶C磷酸化位点、4个酪蛋白激酶Ⅱ磷酸化位点和 3个肉豆蔻酰化位点。　结论　Sjsp16基因编码蛋白为具有脂质识别和结合功能的分泌蛋白 ,可能为潜在的血吸虫病药物靶点或疫苗候选分子。

Cloning and Expression of a Secretory Protein Sjsp16 of Schistosoma japonicum

Objective To clone and express a new secretory protein Sjsp16 of Schistosoma japonicum. Methods The specific primers were designed according to the EST sequence and used for amplification of the encoding sequence from the cDNA clone containing Sjsp16. The gene was subcloned into pET28 plasmid and expressed. The structure and functions of this protein were analysed by bioinformatics method. Results The secretory protein gene of Schistosoma japonicum Sjsp16 was cloned and expressed successfully. The bioinformatics analysis showed that there is a signal peptide at the N terminal, which is the character of a secretory protein. It is predicted that Sjsp16 protein contains ML domain, one N glycosylation site, three phosphorylation sites for protein kinase C, four phosphorylation sites for casein kinase II and three N myristoylation sites. Conclusion The protein encoded by Sjsp16 is a secretory one with lipid recognition and lipid binding function, and might be a new candidate for drug target or vaccine.