丁型肝炎病人肝组织HDAg与HBsAg/HBcAg和HBV DNA表达及关系

目的：探讨丁型肝炎病人肝组织中ＨＤＡｇ与ＨＢｓＡｇ／ＨＢｃＡｇ和ＨＢＶＤＮＡ表达及关系。方法：应用免疫组化双重染色和原位杂交，检测７９例丁型肝炎病人肝组织ＨＤＡｇ、ＨＢｓＡｇ、ＨＢｃＡｇ和ＨＢＶＤＮＡ表达，以５２例乙型肝炎作对照。结果：丁型肝炎ＨＢｓＡｇ、ＨＢｃＡｇ检出率（８１％、７１％）较乙型肝炎（９４％、９２％）低（Ｐ〈０．０５或０．０１）。ＨＤＡｇ以肝细胞核表达为主，ＨＢｓＡｇ以肝细胞浆表达

Relationship among expressions of HDAg, HBsAg/HBcAg and HBV DNA in liver in patients with hepatitis D

Objective:To investigate the relationship among expressions of HDAg, HBs/HBcAg and HBV DNA in the liver in patients with hepatitis D Methods: HDAg, HBsAg, HBcAg and HBV DNA in paraffin embedded liver tissue from 79 patients with hepatitis D were detected with immunohistochemical double labeling and in situ hybridization Meanwhile, they were compared with the expressions of HBsAg and HBcAg in the liver tissues from patients with hepatitis B Results:The detecting rates of HBsAg, HBcAg and HBV DNA in liver specimens from patients with hepatitis D were significantly lower than those in patients with hepatitis B (P< 0 05 or 0 01) HDAg was mainly expressed in nuclei of hepatocytes Much of positive cells were obviously aggregated in periportal region, especially at the advancing edges of areas of piecemeal necrosis Moreover, strong expression of HDAg was seen in the bollooning degenerated hepatocytes and those near piecemeal necrosis region HBsAg was mainly expressed in cytoplasm of hepatocytes and positive cell distribution was similar to that of HDAg positive cells HBcAg was mainly expressed in nuclei of hepatocytes and positive cell distribution was alone for focal type Double labeling showed that both HDAg and HBsAg might be mostly expressed in the same hepatocytes and positive cells were mostly found to locate in the same area of the liver tissue Conclusion:HDV infection can inhibit replication of HBV or expression of HBV antigen Both the interaction between HBV and HDV and the direct cytotoxicity of HDV may play important roles in pathogenesis of hepatitis D