| 邻苯二甲酸二丁酯对体外大鼠肝脏细胞的氧化损伤作用 |
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| 引用本文： | 蔡凤云,王萌,王婧,秦龙娟,杨旭.邻苯二甲酸二丁酯对体外大鼠肝脏细胞的氧化损伤作用[J].湖北预防医学杂志,2009,20(4):4-7. |
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| 作者姓名： | 蔡凤云 王萌 王婧 秦龙娟 杨旭 |
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| 作者单位： | 华中师范大学生命科学学院环境科学实验室和湖北省遗传调控和整合生物学重点实验室; |
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| 基金项目： | 国家科技支撑计划项目(No.2006BAJ02A10;No.2006BAI19B05) |
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| 摘 要： | 目的探讨邻苯二甲酸二丁酯（DBP）对大鼠肝脏细胞的急性氧化损伤作用。方法用0、5、20、80μmo·lL^-1的DBP溶液对大鼠肝脏细胞进行体外染毒1h,然后分别用硫代巴比妥酸（TBA）法和KCl-SDS沉淀法来检测丙二醛（MDA）含量和DNA-蛋白质交联（DPC）系数的变化。结果随着DBP染毒浓度的升高,大鼠肝脏细胞的MDA含量和DPC系数逐渐上升,且在5μmo·lL^-1浓度时就与对照组具有良好的统计学意义（P〈0.01）。结论DBP能够对体外大鼠肝脏细胞造成氧化损伤。
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| 关 键 词： | 邻苯二甲酸二丁酯 肝脏细胞 氧化损伤 丙二醛 DNA-蛋白质交联 |
Oxidative damage of rat liver cells induced by Di-n-butyl Phthalate in vitro |
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| CAI Feng-yun,WANG Meng,WANG Jing,QIN Long-juan,YANG Xu.Oxidative damage of rat liver cells induced by Di-n-butyl Phthalate in vitro[J].Hubei Journal of Preventive Medicine,2009,20(4):4-7. |
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| Authors: | CAI Feng-yun WANG Meng WANG Jing QIN Long-juan YANG Xu |
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| Institution: | CAI Feng-yun,WANG Meng,WANG Jing,QIN Long-juan,YANG Xu. Laboratory of Environmental Sciences , Hubei Key Laboratory of Genetic Regulation , Integrative Biology,College of Life Sciences,Huazhong Normal University,Wuhan 430079,China |
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| Abstract: | Objective The present study was conducted to study the effects of Di-n-butyl phthalate （DBP） on the acute oxidative damage in liver cells of rat. Methods Liver cells of rat were exposed to different concentration of DBP （0, 5,20 and 80 μmo·lL^-1） for one hour in vitro. Thiobarbituric acid （TBA） assay and KCl-SDS assay were used to measure the change of the contents of malondialdehyde （MDA） and the coefficient of DNA-protein crosslinks （DPC） respectively. Results With statistical significance, both MDA contents and DPC coefficient in liver cells increased gradually with the increase of DBP concentrations. Conclusion These results suggested that DBP could induce the acute oxidative damage in liver cells of rat. |
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| Keywords: | Di-n-butyl phthalate （DBP） Liver cells Oxidative damage Malondialdehyde （MDA） DNA-protein crosslinks （DPC） |
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