四种肝病相关自身抗体联合检测在肝功能异常血清中的临床价值

目的探讨4种肝病相关自身抗体在肝功能异常患者血清中的阳性率及其诊断价值。方法间接免疫荧光法检测血清中ANA、SMA、AMA、抗LKM-I，根据荧光反应强弱判定结果。结果150例肝功异常患者（ALT〉50U／L）自身抗体阳性率为18.7％（28／150），其中抗核抗体（ANA）、抗平滑肌抗体（SMA）及抗线粒体抗体（AMA）的阳性率分别为12.7％（19／150）、6.7％（10）／150）和7.3％（11／150）；未检出抗肝肾微粒体I型抗体（LKM-I）；女性患者自身抗体的检出率明显高于男性患者（P〈0.05）：病毒性肝炎标志物阴性组自身抗体检出率明显高于阳性组（P〈0.05）。结论自身抗体对自身免疫性肝病在诊断疾病、了解病程和观察疗效中具有很重要的价值，肝功能异常的非病毒性肝炎病人适合进行肝病相关自身抗体的检测。

Analysis on the co-detection of autoantibodies in patients with abnormal liver function

Objective To investigate the positive rates of four autoantibodies in patients with abnormal liver function and explore the clinical significance of the autoantibodies related to liver diseases. Methods 150 patients with abnormal liver function were detected for ANA, SMA, anti-LKM-I and AMA using indirect immunofluorescence. Results of 18% positive rates of autoantibodies, ANA, SMA, and SMA were 12.7%, 6.7% and 7.3%, and the level of female group was higher than that of male group （P〈0.05）, non-viral hepatitis group was higher than those of viral hepatitis group （P〈0.05）. Conclusions The detection of autoantibodies may be useful for the diagnosis of autoimmune liver diseases. The non-viral hepatitis patients with abnormal liver function are suitable for detecting hipatitis-associated autoantibodies.