榆黄喷雾剂质量标准的建立

目的：本研究旨在建立榆黄喷雾剂的质量标准。方法：采用薄层色谱法与紫外分光光度法对榆黄喷雾剂中的盐酸小檗碱进行定性鉴别及含量测定,通过香草醛硫酸法测定缩合鞣质。结果：榆黄喷雾剂中盐酸小檗碱的薄层色谱显示,在与对照品色谱相应的位置上,显相同颜色的斑点,阴性无干扰;紫外分光光度法测定盐酸小檗碱,盐酸小檗碱在1.13-13.60μg·m^l-1之间呈良好线性关系（r=0.999 9）,平均回收率为99.68%,RSD=1.53%（n=9）;香草醛硫酸法测定榆黄喷雾剂中缩合鞣质含量,儿茶素对照品浓度在0.03-0.30 mg·ml^-1范围内与吸收度具有很好的线性关系r=0.999 3,平均回收率为99.6%,RSD为1.76%（n=9）。结论：研究证明,该方法可用于榆黄喷雾剂的质量控制。

Quality Standard for Yuhuang Sprays

Objective： To establish the quality standard for Yuhuang sprays. Methods： Berberine hydrochloride in Yuhuang sprays was qualitatively identified by TLC and determined by UV. The Vanillin-H2 SO4 method was employed for the determination of condensed tannins. Results：The spots obtained from the sample solutions showed the same color as those from the reference substance solution without interference from the negative sample. The calibration curve of berberiny hydrochloride was linear within the range of 1. 13-13. 60μg·ml-1（r=0. 999 9）, and the average recovery was 99. 68% with RSD of 1. 53%（n=9）. The calibration of catechin was linear within the range of 0. 03-0. 30 mg· ml-1（r=0. 999 3）, and the average recovery was 99. 6% with RSD of 1. 76%（n=9）. Conclusion：The method can be used in the quality control of Yuhuang sprays.