HPLC-RID法测定转化糖电解质注射液中果糖和葡萄糖含量

目的：建立高效液相色谱法通过示差折光检测器同时测定转化糖电解质注射液中果糖和葡萄糖含量。方法：色谱柱为Waters XbridgeTMAmide（150 mm×4.6 mm,3.5μm）,以乙腈-水-三乙胺（75∶25∶0.2）为流动相,柱温为40℃;流速：1.0 ml·min^-1;示差折光检测器,检测器温度为40℃,进样量：50μl。结果：果糖在99.8-149.7μg（r=0.999 7）、葡萄糖在100.3-150.4μg（r=0.999 2）范围内与峰面积呈良好的线性关系;果糖和葡萄糖的平均回收率分别为99.2%、99.5%,RSD分别为0.81%、0.84%（n=9）。结论：此法专属性较高,准确度良好且方便快捷,分离效果好,可用于同时测定果糖和葡萄糖的含量。

Determination of Fructose and Glucose in Multiple Electrolytes and Invert Sugar Injections by HPLC with Refractive Index Detector

Objective：To establish an HPLC method with a refractive index detector for the simultaneous determination of fructose and glucose in multiple electrolytes and invert sugar injections. Methods： A Waters XbridgeTM Amide（150 mm × 4. 6 mm,3. 5 μm） column was used with acetonitrile-water -triethylamine （75∶25∶0. 2） as the mobile phase, the column temperature was 40 ℃, the flow rate was 1. 0 ml·min-1 , the refractive index detector was used with the detector temperature of 40 ℃, and the injection volume was 50 μl. Results：A good linear relationship between the quantity of glucose or fructose and peak area was shown within the range of 99. 8-149. 7 μg（r=0. 999 7）for fructose and 100. 3-150. 4 μg（r=0. 999 2）for glucose, the average recovery of fructose and glucose was 99. 2% and 99. 5% with RSD of 0. 81% and 0. 84%（n=9）, respectively. Conclusion： The method is fast and convenient with high specificity, good accuracy and promising separation, which can be used in the content determination of fructose and glucose in multiple electrolytes and invert sugar injections.