Genomovar status, virulence markers and genotyping of Burkholderia cepacia complex strains isolated from Brazilian cystic fibrosis patients |
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| Authors: | K tia Maia Martins, Giuliana F. Fongaro, Andr B. Dutra Rodrigues, Adriana F. Tateno, Ana Carolina Azzuz-Chernishev, Doroti de Oliveira-Garcia, Joaquim C. Rodrigues,Luiz Vicente F. da Silva Filho |
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| Affiliation: | 1. Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil;2. Bacteriology Section, Adolfo Lutz Institute, São Paulo, Brazil;3. Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil |
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| Abstract: | Burkholderia cepacia complex isolates obtained by microbiological culture of respiratory samples from Brazilian CF patients were studied by recA based PCR, screened by specific PCR for virulence markers and genotyped by RAPD. Forty-one isolates of B. cepacia complex were identified by culture and confirmation of identity and genomovar determination obtained in 32 isolates, with predominance of B. cenocepacia (53.1%). Virulence markers were not consistently found among isolates. Genotyping did not identify identical patterns among different patients. B. cenocepacia was the most prevalent B. cepacia complex member among our patients, and cross-infection does not seem to occur among them. |
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| Keywords: | Burkholderia cepacia complex Cystic fibrosis Children Virulence factors Molecular epidemiology |
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