大鼠脑缺血后PEDF表达及替米沙坦调控作用的研究

目的动态观察大鼠脑缺血后色素上皮源性生长因子(PEDF)的表达变化,并探讨替米沙坦的调控机制。方法线栓法建立大脑中动脉闭塞(MCAO)大鼠模型。实验1,动态观察PEDF蛋白和基因表达;实验2,替米沙坦干预,采用Western blot和qR-T PCR观察PEDF基因和蛋白变化,比较各组脑梗死体积、患侧脑水肿和神经功能。结果 MCAO后24h开始,PEDF表达明显减少。替米沙坦通过激活过氧化物酶体增殖物激活受体γ(PPARγ)上调PEDF,明显改善神经功能缺失,减轻脑水肿,减小梗死体积。结论调控内生性PEDF表达可能是治疗脑缺血的新靶点。

The intrinsic PEDF expression after focal cerebral ischemia in rats and the regulation of telmisartan

Objective This study is to evaluate the time course expression of PEDF and explore the underling regulation mechanisms of telmisartan.Methods Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion(MCAO).Experiment 1: The time course expression of PEDF protein and gene was evaluated.Experiment 2: Telmisartan was systemically administered to explore the effect on PEDF at 24 h after cerebral ischemia by western blot and qRT-PCR.The neurological deficits,brain water content and infarct volume were measured.Results Telmisartan dramatically up-regulated PEDF,alleviated the neurological deficits,brain water content and infarct volume in PPARγ-dependent way.Conclusion The regulation of intrinsic PEDF may be one of the strategic targets for cerebral ischemic therapies.