DLC2和RhoA蛋白在原发性肝癌组织中的表达及其临床意义

目的:探讨肝癌缺失基因2(DLC2)和RhoA蛋白在肝癌组织中的表达及其与预后的关系。方法:采用Western印迹法和免疫组化技术检测128例原发性肝癌组织中DLC2蛋白和RhoA蛋白的表达;分析DLC2蛋白的表达与肝癌临床病理特征的关系及DLC2蛋白对原发性肝癌预后的价值。结果:DLC2蛋白在HCC组织中的表达较癌旁组织低,差异具有统计学意义(P<0.05)。DLC2蛋白在细胞分化程度差的肝癌组织中表达降低(P<0.05)。同时,DLC2蛋白的低表达与RhoA蛋白的高表达呈正相关。DLC2蛋白表达低的原发性肝癌患者预后更差。结论:原发性肝癌组织中DLC2蛋白的低表达与较差的细胞分化程度,RhoA蛋白的高表达相关,DLC2缺失表达的原发性肝癌患者预后较差。

Underexpression of deleted in liver cancer 2 (DLC2) is associated with overexpression of RhoA and poor prognosis in hepatocellular carcinoma

Objective: To investigate the DLC2 protein expression and its correlation with expression of RhoA and prognosis in hepatocellular carcinoma (HCC) patients.Methods: Western blotting and immunohistochemical staining were employed to detect DLC2 protein expression in 128 HCC specimens. The correlation between DLC2 protein expression and clinicopathological characteristics and prognostic value of DLC2 for HCC patients were analyzed. Results: HCC tissues revealed significantly lower level of DLC2 protein than pericarcinomatous liver tissues (PCLT) (P<0.05). There was a significant correlation between underexpression of DLC2 protein and cell differentiation (P<0.05). Meanwhile, underexpression of DLC2 protein was correlated with overexression of RhoA. Furthermore, HCC patients with DLC2-negative expression showed a significantly poorer prognosis than those with DLC2-positve expression.Conclusion: Our data indicates that decreased DLC2 expression in HCC correlates with cell differentiation of HCC and overexpression of RhoA, and underexpression of DLC2 is associated with poor prognosis in HCC patients.