白细胞介素1B-511多态性与侵袭性牙周炎的关系

目的研究中国人群中IL-1B-511位点单核苷酸多态性(single nucleotide polymorphism,SNP)与侵袭性牙周炎(aggressive periodontitis,AgP)之间的关系.方法提取122名AgP患者和95名健康对照者外周静脉血基因组DNA,应用聚合酶链反应-限制性片段长度多态性的方法,分析IL-1B-511位点SNP与AgP的关联性.结果男性AgP组杂合基因型的频率较男性健康对照组升高并有统计学意义(P=0.048);A2 基因型/等位基因A2与吸烟联合作用显著增加了AgP的易感性(基因型:P=0.022;等位基因:P=0.006).结论IL-1B-511位点的SNP可能与中国人群中男性个体的AgP易感性有关;该位点的SNP与吸烟可能对AgP的易感性具有联合效应.

Association analysis between Interleukin- 1B - 511 polymorphism and aggressive periodontitis

Objectives:To study whether Interleukin-1B (IL-1B) -511 genotype and/or allele were associated with aggressive periodontitis (AgP) in Chinese.Methods: AgP group consisted of 122 patients and healthy controls group consisted of 95 subjects. Single nucleotide polymorphism at IL-1B -511 was analyzed by standard Polymerase Chain Reaction-Restriction Fragment Length Polymorphism assay. Results: The frequency of IL-1B -511 A1/A2 heterozygote was significantly increased in male AgP group compared to male control (P = 0.048). The adjusted OR of AgP for combined A2+ genotype and smoking was 12.45 (P = 0.022), and for combined allele 2 and smoking was 18.25 (P = 0.006).Conclusion: The IL-1B -511 polymorphism may play an important role in determining AgP susceptibility in Chinese male. A possible combined effect of the IL-1B -511 polymorphism and smoking on AgP susceptibility was suggested.