缺血预处理对肢体缺血-再灌注损伤的保护作用

目的 :探索缺血预处理对肢体再灌注损伤的保护作用。方法 :分别给予兔后肢一次或多次短暂的缺血预处理 ,然后于持续缺血 4h ,再灌注 1h后行股静脉穿刺 ,抽血检测血清肌磷酸激酶 (CPK)、谷草转氨酶 (GOT)、乳酸脱氢酶 (LDH)及超氧化物歧化酶 (SOD)和丙二醛 (MDA)含量 ,并取腓肠肌病检。结果 :缺血预处理各组再灌注后肌细胞内酶释放减少 ,病理切片也发现骨骼肌坏死和梗塞灶减少 ,血清MDA含量下降 ,而SOD活力升高。不同次数的缺血预处理结果之间存在差异。结论 :缺血预处理可以影响体内的自由基代谢 ,对肢体再灌注损伤具有保护作用 ,且这种保护作用与预处理次数有一定关系。

Protective effect of ischemic preconditioning on ischemia-reperfusion injury

Objective: To study the protective effect of ischemic preconditioning(IP) on ischemia reperfusion injury of limb. Methods: One to three times of short term ischemia was applicated before prolonged ischemia, a series of biological examinations, including creatine phosphate kinase(CPK), glutamic oxalacetic transaminase(GOT), lactic dehydrogenase(LDH), malondiadehyde(MDA) and superoxide dismutase(SOD) were carried out in each group. Results: Compared with the control, release of skeletal muscle intracellular enzyme(CPK, GOT and LDH) decreased in experiment groups after preconditioning had been performed. The serum concentration of MDA was lower, activity of SOD was higher, and pathological lesion was less in preconditioning groups than that in non preconditioning group. Different frequency of preconditioning led to different results. Conclusions: Preconditioning has protective effect on ischemia reperfusion injury of limbs, and the effect of IP is correlated with the number of preconditioning cycles.