| 缺血再灌注大鼠肠上皮细胞线粒体DNA ATPase 6,8亚基基因的改变 |
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| 引用本文： | 李伟文,陆松敏,刘建仓,武凡,柏干荣,李萍.缺血再灌注大鼠肠上皮细胞线粒体DNA ATPase 6,8亚基基因的改变[J].中国现代医学杂志,2004,14(20):23-26,29. |
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| 作者姓名： | 李伟文 陆松敏 刘建仓 武凡 柏干荣 李萍 |
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| 作者单位： | 第三军医大学附属大坪医院野战外科研究所第二研究室,重庆,400042 |
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| 摘 要： | 目的观察缺血再灌注大鼠肠上皮细胞mtDNA ATPase 6,8亚基基因的改变情况.方法将6只Wistar大鼠分成对照组、缺血再灌注组.分别提取mtDNA,采用PCR技术扩增后测序,检测突变点.结果 Wistar大鼠mtDNA ATPase 6,8亚基基因存在多态性,在缺血再灌注组存在a7797缺失和一定数量点突变:A7 779T,T8 515G,T8 520G,C8 535G.结论缺血再灌注后,肠上皮细胞mtDNA ATPase 6,8亚基基因存在损伤,由此可能导致所编码的氨基酸改变.
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| 关 键 词： | 早期复苏 线粒体DNA ATPase 6基因 ATPase 8基因 多态性 突变 |
Study on changes of mitochondrial DNA ATPase 6,8 genes of small intestine epithelial cells in ischemia-reperfusion rats |
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| Abstract.Study on changes of mitochondrial DNA ATPase 6,8 genes of small intestine epithelial cells in ischemia-reperfusion rats[J].China Journal of Modern Medicine,2004,14(20):23-26,29. |
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| Authors: | Abstract |
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| Abstract: | Objective: To observe the changes of mitochondrial DNA ATPase 6, 8 genes of small intestine epithelial cells in ischemia-reperfusion rats. Methods: 6 rats were randomly divided into the control and ischemia-reperfusion group. Their mtDNA were distilled and the sequences were examined after polymerase chain reaction conducted by using special primer in order to detect mutational sites. Results: The polymorphism existed at some sites of ATPase 6, 8 genes of Wistar rats in China. There was a A7 797 deletion and several sites mutation of ATPase 6, 8 genes in ischemia-reperfusion group, namely, A7 779T, T8 515G, T8 520G, C8 535G. Conclusion: The damage of ATPase 6, 8 genes took place after ischemia-reperfusion and this may result in the changes of their coding amino acids. |
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| Keywords: | ischemia-reperfusion mtDNA ATPase 6 gene ATPase 8 gene Polymorphism mutation |
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