反相高效液相色谱法测定重楼分散片中重楼皂苷Ⅰ的含量

目的建立反相高效液相色谱法测定重楼分散片中重楼皂苷Ⅰ含量方法，为控制重楼分散片的质量提供依据。方法色谱柱为Waters Symmetry Shield TMRP18柱，柱温为室温，流速为1．0mL／min，检测波长为203nm，以乙腈-水（41：59）为流动相，室温操作。结果该方法线性范围为2．112-21.112μg，r=0．9999，平均回收率为99．8％（RSD=1．48％，n=6）。结论反相高效液相色谱法测定重楼分散片中重楼皂苷Ⅰ含量简便、准确，结果稳定，重复性好。

Determination of Parissaponin I in Gongxuening Oliv Dispersible Tablets by RP-HPLC

Objective To establish a method of reverse phase high pressure liquid chromatography （RP-HPLC） for determining the parissaponin Ⅰ content in gongxuening oliv dispersible tablets. Methods Hypersil Waters Symmetry Shield TMRP18 column was selected as the medium for analyzing the samples at room temperature,and acetonitrile-water（41 ： 59）as the mobile phase,and the velocity of flow was l. 0 mL/min,the detecting wavelength was 203 nm. Results The standard curve was linear at the range of 2. 112-21. 112 μg （r=0. 999 9）,the average recovery was 99.8-（RSD=1. 48%,n=6）. Conclusion RP-HPLC is sensitive,accurate,simple,and repeatable method for detecting parissaponin gongxuening oliv dispersible tablets; parissaponin Ⅰ; RP-HPLC content in gongxuening oliv dispersible tablets.