Structural polypeptides of the enteropathogenic bovine coronavirus strain LY-138 |
| |
| Authors: | Dr I Hajer J Storz |
| |
| Institution: | (1) Present address: Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA;(2) Present address: the Department of Virology, Veterinary Research Administration, El Amarat, Khartoum, Sudan;(3) Institut für Virologie, Justus Liebig-Universität Giessen, Frankfurter Strasse 107, D-6300 Giessen, Federal Republic of Germany |
| |
| Abstract: | Summary The bovine coronavirus strain LY-138 was purified by differential as well as velocity and isopycnic centrifugation in sucrose or CsCl gradients. The substrate for purification was contents of the small intestine of experimentally inoculated calves. This strain is highly enteropathogenic, but it could not yet be propagated in cultured cells. Intact virions had a density of 1.245 g/cm3 in CsCl and 1.185 g/cm3 in sucrose. A spherical core-like structure with an average diameter of 82 nm remaining after treatment with chloroform had a density of 1.299 g/cm3 in CsCl and 1.201 g/cm3 in sucrose.Seven distinct bands of polypeptides and 4 shoulders were detected after electrophoresis of SDS-solubilized virions in polyacrylamide gels. The approximate molecular weights ranged from 110,000 to 36,000. Four of the bands gave a PAS positive reaction. These 4 glycoproteins and an additional protein with an approximate molecular weight of 70,000 were removed by chloroform treatment. The remaining core-like structure contained the 2 polypeptides VP3 and VP7.With 5 Figures |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
