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| 柴胡中柴胡总皂苷及柴胡皂苷a的含量测定 | |
| 引用本文： | 朱兰兰,孙玲,翟丽屏,黄伟,李素君,陆永辉,孙蓉.柴胡中柴胡总皂苷及柴胡皂苷a的含量测定[J].中国药物警戒,2011,8(8):465-467. |
| 作者姓名： | 朱兰兰 孙玲 翟丽屏 黄伟 李素君 陆永辉 孙蓉 |
| 作者单位： | 1. 山东省中医药研究院,山东,济南,250014;天津中医药大学,天津,300193 2. 山东省医药工业研究所,山东,济南,250101 3. 山东省地方病研究所,山东,济南,250014 4. 山东省中医药研究院,山东,济南,250014 5. 山东中医药大学,山东,济南,250355 |
| 基金项目： | 国家自然科学基金课题，山东省引进国外智力项目，山东省科技攻关关键技术研究课题，山东省科技平台建设项目 |
| 摘 要： | 目的 建立柴胡中柴胡总皂苷及柴胡皂苷a的含量测定方法,并对样品进行含量测定.方法 采用紫外分光光度法,以柴胡皂苷a为对照品,在波长545nm处对样品中的总皂苷进行含量测定;采用高效液相色谱法,以C18色谱柱(4.6mmX250mm.5μm)、甲醇-水为流动相,流速为1mL·min<'-1>,检测波长为210nm,测定样品中柴胡皂苷a的含量.结果 柴胡总皂苷在30～70μg/mL、柴胡皂苷a在50.4～252μg/mL范围内呈良好的线性关系,r分别为09956、0.9991;平均回收率:柴胡总皂苷为99.31%、柴胡皂苷a为99.22%;RSD:柴胡总皂苷为1.25%,柴胡皂苷a为1.07%.结论 本研究所建立的紫外分光光度法测定柴胡总皂苷及高效液相法测定皂苷a含量的方法,简便、易行、快速,结果准确可靠,适用于柴胡中柴胡总皂苷及柴胡皂苷a的含量测定,并为在安全剂量范围内正确使用柴胡提供依据. |
| 关 键 词： | 柴胡总皂苷 柴胡皂苷a 含量测定 |
Determination of the Content of Saikosaponins and Saikosaponin a in Bupleurum Chinense DC. |
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| ZHU Lan-lan,SUN Ling,ZHAI Li-ping,HUANG Wei,LI Su-jun,LU Yong-hui,SUN Rong.Determination of the Content of Saikosaponins and Saikosaponin a in Bupleurum Chinense DC.[J].Chinese JOurnal of Pharmacovigilance,2011,8(8):465-467. | |
| Authors: | ZHU Lan-lan SUN Ling ZHAI Li-ping HUANG Wei LI Su-jun LU Yong-hui SUN Rong |
| Institution: | ZHU Lan-lan1,2 SUN Ling3 ZHAI Li-ping4 HUANG Wei1 LI Su-jun5 LU Yong-hui1 SUN Rong1(1Shandong Academy of Chinese Medicine,Shandong Jinan 25014,China,2Tianjin University of Traditional Chinese Medicine,Tianjin 300193,3Shandong Institute of Pharmaceutical Industry,Shandong Jinan 250101,4Shandong Institute for Prevention and Treatment of Endemic Disease,Shandong Jinan 250014,5Shandong University of Traditional Chinese Medicine,Shandong Jinan 250355,China) |
| Abstract: | Objective To establish the method for determination of the content of saikosaponins and saikosaponin a in Bupleurum Chinense DC,and carries on determination of the content of the sample of saikosaponins and saikosaponin a.Methods Saikosaponin a was used as the chemical reference substance determine the content of saikosaponins by UV-Visible Spectrophotometry.The absorbency was measured at 545nm.The content of saikosaponin a in Bupleurum Chinense DC was determined by HPLC.The samples were separated by C18 co... |
| Keywords: | Saikosaponins Saikosaponin a determination |
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