郴州地区汉族人群ACE基因I／D多态性与2型糖尿病的关系

目的评估郴州地区汉族人群血管紧张素转化酶（angiotensin-convertingenzyme，ACE）基因插入／缺失（insertion／deletion，I／D）多态性与2型糖尿病的关系。方法采用横断面研究方法，随机纳入340例对象，其中2型糖尿病患者128例，前糖尿病患者100例，非糖尿病者112例。用PCR法检测ACE基因I／D多态性，基因型分为II、ID或DD型。结果三组患者的年龄（χ2=32．09，P〈0．01）、BMI（χ2=16．10，P〈0．01）、糖尿病家族史（χ2=20．42，P〈0．01）和血清甘油三酯水平（F=21．289，P〈0．01）差异有统计学意义。三种基因型II、ID和DD的分布在2型糖尿病组为72例（56．3％），52（40．6％）和4例（3．1％）；在前糖尿病组为64例（64．0％），30例（30．0％）和6例（6．0％）；在非糖尿病组为81例（72．3％），29例（25．9％）和2例（1．8％）例。ID＋DD基因型与II基因型2型糖尿病的患病比例差异有统计学意义（X2=6．68，P〈0．05）。logistic回归分析表明2型糖尿病的危险因素包括ACE基因I／D多态性、糖尿病家族史、BMI和血清甘油三酯浓度。结论ACE基因ID＋DD基因型与郴州地区社区汉族人群2型糖尿病的发病相关。

The association between ACE gene insertion/deletion polymorphism and type 2 diabetes in Han Chinese from Chenzhou area

Objective To determine the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism in Han ethnic patients with type 2 diabetes in Chenzhou area of Hunan province,and to assess the assoeiation between this polymorphism and diabetes.Methods This study was cross-sectional designed.Samples were taken by random sampling method from diabetes, prediabetes and euglycemia subjeers.ACE gene polymorphism I/D were examined by PCR.Genotype was classified as I1, ID, or DD based on positive or negative insertion/deletion allele.Results This study showed significant differences of ages (X2 =32.09, P ＜0.01), body mass index (BMI) (X2 =16.10, P ＜0.01), family history (X2 =20.42, P ＜0.01) and serum triglyceride (F =21.289, P ＜0.01) among three groups, and diabetes group tended to have higher value.Frequency of genotype II, ID, DD were 72(56.3％) ,52(40.6％) and 4(3.1％) in diabetes, 64(64.0％) ,30(30.0％) and 6(6.0％) in pre-diabetes subjects, 81 (72.3％),29(25.9％)和 2(1.8％) in euglycemia subjects, consecutively.Chi-square analysis showed statistically significant association between ID + DD vs.Ⅱ genotype and diabetes (x2 = 6.68, P ＜ 0.05).Logistic regression analysis showed four variables that significantly influence to diabetes, namely ACE gene polymorphism, family history of diabetes, BMI, and serum triglyceride level.Conclusions ACE ID + DD genotype had significant relationship with diabetes in Chenzhou Han ethnic population, Hunan province, China.