| 高效液相色谱法测定石杉碱甲缓释贴片中石杉碱甲的含量 |
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| 引用本文： | 吴婷,张军,储继红,居文政,许美娟,陈玟,谈恒山. 高效液相色谱法测定石杉碱甲缓释贴片中石杉碱甲的含量[J]. 中国药物与临床, 2011, 11(9): 1000-1002 |
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| 作者姓名： | 吴婷 张军 储继红 居文政 许美娟 陈玟 谈恒山 |
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| 作者单位： | 1. 南京中医药大学附属医院临床药理科,210029
2. 南京军区总医院临床药理科 |
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| 摘 要： | 目的 测定石杉碱甲缓释贴片中石杉碱甲的含量.方法 采用高效液相色谱法(HPLC),Agilent Zorbax Ehipse DXB-C18柱,甲醇-0.8%三乙胺溶液(pH 10.5)(体积比55∶45)为流动相,检测波长为313 nm,流速为1.0 ml/min,柱温为30 ℃,进样量20 μl.结果 石杉碱甲在0.155～2.472 μg/ml范围内有良好的线性关系(r＝0.9996).平均回收率为96.44%,相对标准差(RSD)＝1.14%.结论 本方法简便、准确、专属性强,可用于该制剂的质量控制.
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| 关 键 词： | 色谱法,高压液相 迟效制剂 质量控制 石杉碱甲 |
Determination of huperzine A in huperzine A sustained-release patches by high performance liquid chromatography (HPLC) |
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| WU Ting,ZHANG Jun,CHU Ji-hong,JU Wen-zheng,XU Mei-juan,CHEN Min,TAN Heng-shan. Determination of huperzine A in huperzine A sustained-release patches by high performance liquid chromatography (HPLC)[J]. Chinese Remedies & Clinics, 2011, 11(9): 1000-1002 |
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| Authors: | WU Ting ZHANG Jun CHU Ji-hong JU Wen-zheng XU Mei-juan CHEN Min TAN Heng-shan |
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| Affiliation: | WU Ting,ZHANG Jun,CHU Ji-hong,JU Wen-zheng,XU Mei-juan,CHEN Min,TAN Heng-shan.Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Jiangsu 210029,China |
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| Abstract: | Objective To determine the content ot huperzme A m nuperzme a sustalneo-release patcnes. Methods The huperzine A sustained-release patches were detected by high performance liquid chromatography （HPLC） on Agilent Zorbax Ehipse DXB-C18 column with 0.8% triethylamine （pH 10.5, V/V 55：45） as the mobile phase, a detection wavelength of 313 nm, a flow rate of 1.0 mL/min, a column temperature of 30 ~C and a sample amount of 20 p,L. Results The huperzine A showed a favorable linearity in a range from 0.155 to 2.472 μg/ml （r=0.9996）. The mean recovery was 96.44% （RSD=l.14%）. Conclusion The method in this study appeared simple and exclusively accurate, which justifies its use in quality control of huperzine A preparations. |
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| Keywords: | Chromatography high pressure liquid Delayed-action preparations Quality control Huperzine A |
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