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| 凝胶滞留电泳法检测人宫颈鳞癌细胞核中ETS1结合活性 | |
| 引用本文： | 王晓燕,张宇涛,赵霞,赵瑞霞. 凝胶滞留电泳法检测人宫颈鳞癌细胞核中ETS1结合活性[J]. 中国药物与临床, 2011, 11(10): 1139-1141 |
| 作者姓名： | 王晓燕 张宇涛 赵霞 赵瑞霞 |
| 作者单位： | 1. 山西省肿瘤医院妇科,太原,030013 2. 大同市第三医院妇产科 3. 山西省疾病预防控制中心理化检验科 |
| 基金项目： | 山西省卫生厅科技攻关项目 |
| 摘 要： | 目的应用人宫颈鳞癌组织细胞核蛋白提取物与特异ETS1基因探针序列结合,经凝胶滞留电泳(EMSA)分析其结合能力。初步探讨ETS1作为转录因子在人宫颈鳞癌发生、进展中的作用机制。方法通过EMSA,应用探针标记技术,在转录水平进一步揭示了ETS1结合DNA能力的调控作用。结果随着宫颈鳞癌的发生发展,ETS1结合DNA的能力增强,EMSA中探针迁移率明显降低(P<0.01)。结论 ETS1与宫颈癌发生发展有相关性,其与肿瘤浸润和转移有密切的关系。 |
| 关 键 词： | 宫颈肿瘤 电泳 基因 |
Expression of ETSI in invasive uterine cervical cancer and its significance |
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| WANG Xiao-yan,ZHANG Yu-tao,ZHAO Xia,ZHAO Rui-xia. Expression of ETSI in invasive uterine cervical cancer and its significance[J]. Chinese Remedies & Clinics, 2011, 11(10): 1139-1141 | |
| Authors: | WANG Xiao-yan ZHANG Yu-tao ZHAO Xia ZHAO Rui-xia |
| Affiliation: | WANG Xiao-yan*,ZHANG Yu-tao,ZHAO Xia,ZHAO Rui-xia.*Department of Gynecology,Shanxi Provincial Tumor Hospital,Taiyuan 030013,China |
| Abstract: | Objective To investigate the role of ETS1 as a transcription factor in development and progression of human cervical cancer by binding ETS1 gene-specific probe to protein concentrate from cervical cancer on electrophoretic mobility shift assay(EMSA).Methods Binding of ETS1 proteins to DNA was examined with specific probes and EMSA,which would shed light on the regulatory activity of ETS1 at the level of transcription.Results EMSA showed higher DNA-binding ability of ETS1 along with progression of cervical l... |
| Keywords: | Gervix neoplasms Electrophoresis Genes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |