六味地黄苷糖中糖苷部位的化学成分研究

目的:对六味地黄苷糖中糖苷部位的化学成分进行研究,阐明其活性的物质基础.方法:用大孔树脂柱色谱制备糖苷部位,用硅胶和反相硅胶柱色谱分离各成分,用MS及NMR技术鉴定结构.结果:分离得到了11个化合物,其中包括9个环烯醚萜苷,1个蒎烷单萜苷,1个酚苷,分别鉴定为7-脱氢马钱素(1),7α-甲氧基莫诺苷(2),7β-甲氧基莫诺苷(3),7α-乙氧基莫诺苷(4),7β-乙氧基莫诺苷(5),莫诺苷(6),獐芽菜苷(7),马钱素(8),芍药苷(9),丹皮酚原苷(10),番木鳖酸(11).结论:该糖苷部位是新药六味地黄苷糖发挥免疫和内分泌调节作用的重要部分.化合物6～8是该部位的主要成分,具有较好的免疫调节作用,是该部位主要活性成分.

Chemical constituents of glucoside fraction from Liuwei Dihuang Gantang

Objective: To study chemical constituents of glucoside fraction from Liuwei Dihuang Gantang and clarify its substance foundation of active constituents.Method: Glucoside fraction was prepared by macroporous resin chromatography.Constituents were separated by silica gel and reverse phase silica gel column chromatography,and their structures were identified by MS and NMR.Result: Eleven compounds were separated and identified as 7-dehydrologanin(1),7α-O-methylmorroniside(2),7β-O-methylmorroniside(3),7α-O-ethylmorroniside(4),7β-O-ethylmorroniside(5),morroniside(6),sweroside(7),loganin(8),paeoniflorin(9),paeonolide(10) and loganic acid(11).Conclusion: All of those compounds were separated from Liuwei Dihuang Gantang for the first time.