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| 四氯化碳药物性肝损伤体外模型的改进 | |
| 引用本文： | 吴海建,龚秀,杨倚天,王玉卓,李霞,辛华,胡立宽. 四氯化碳药物性肝损伤体外模型的改进[J]. 中国中药杂志, 2012, 37(23): 3633-3636 |
| 作者姓名： | 吴海建 龚秀 杨倚天 王玉卓 李霞 辛华 胡立宽 |
| 作者单位： | 1. 山东大学齐鲁医院肿瘤中心,山东济南250012;山东大学医学院,山东济南250012 2. 复旦大学华山医院,上海,200040 3. 北京301医院介入放射科,北京,100853 4. 山东大学医学院,山东济南,250012 |
| 摘 要： | 目的:建立一个较理想的CCl4药物性肝损伤体外模型.方法:分别采用传统方法和改进方法配制CCl4损伤液并诱导人肝HepG2细胞损伤,倒置相差显微镜观察细胞形态学变化,生化法检测上清液中ALT水平并采用MTT法测定细胞活性.结果:改进方法的CCl4诱导损伤效果明显优于传统方法,随着CCl4损伤液浓度的提高,上清液中ALT水平明显升高,而细胞活性显著降低,70％浓度CCl4损伤液诱导损伤4h可获得最佳损伤效果.结论:利用改进方法可在体外实验中建立较理想的CCl4药物性肝损伤模型,此模型可为进一步的体外实验研究奠定基础. |
| 关 键 词： | 四氯化碳 HepG2细胞 药物性肝损伤 |
| 收稿时间： | 2012-03-09 |
Improvement of carbon tetrachloride drug-induced liver injury model in vitro |
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| WU Hai-jian,GONG Xiu,YANG Yi-tian,WANG Yu-zhuo,LI Xi,XIN Hua and HU Li-kuan. Improvement of carbon tetrachloride drug-induced liver injury model in vitro[J]. China Journal of Chinese Materia Medica, 2012, 37(23): 3633-3636 | |
| Authors: | WU Hai-jian GONG Xiu YANG Yi-tian WANG Yu-zhuo LI Xi XIN Hua HU Li-kuan |
| Affiliation: | Cancer Center, Qilu Hospital, Shandong University, Ji'nan 250012, China;School of Medicine, Shandong University, Ji'nan 250012, China;Huashan Hospital, Fudan University, Shanghai 200040, China;Interventional Radiology, Beijing 301 Hospital, Beijing 100853, China;School of Medicine, Shandong University, Ji'nan 250012, China;School of Medicine, Shandong University, Ji'nan 250012, China;School of Medicine, Shandong University, Ji'nan 250012, China;Cancer Center, Qilu Hospital, Shandong University, Ji'nan 250012, China;School of Medicine, Shandong University, Ji'nan 250012, China |
| Abstract: | Objective: To establish an ideal CCl4 drug-induced liver injury model in vitro. Method: Traditional method and improved method were adopted for preparing CCl4 injury liquid and drug-induced human liver HepG2 cell injury. Cell morphological change was observed under a bright-field microscope. The level of alanine aminotransferase (ALT) in supernatant was detected by biochemical method. 4-Methyl-tetrazolium (MTT) chromatometry was adopted for determining cell activity. Result: The improved method showed better CCl4-induced injury effect than the traditional method. With the increase in the concentration of CCl4 injury liquid, the ALT level significantly increased, whereas the cell activity notably decreased. Particularly, 70% CCl4 injury liquid use for 4 hours could achieve the best injury effect. Conclusion: The improved method could be used to establish an ideal CCl4 drug-induced liver injury model in vitro, which can lay foundation for further in vitro studies. |
| Keywords: | carbon tetrachloride HepG2 cell drug-induced liver injury |
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