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Comparison of several procedures for the preparation of synaptosomal plasma membrane vesicles |
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| Authors: | Il Yun Young-Shin Kim Seong-Ho Yu In-Kyo Chung In-Se Kim Seung-Wan Baik Goon-Jae Cho Yong-Za Chung Seok-Hwan Kim Jung-Sook Kang |
| Affiliation: | 1. Department of Oral Biology and Biophysics, College of Dentistry, Pusan National University, 602-061, Pusan, Korea 2. Department of Oral Biology, College of Dentistry, Pusan National University, 602-061, Pusan, Korea 3. Department of Oral and Maxillofacial Surgery and Clinical Pharmacology Unit, College of Dentistry, Pusan National University, 602-061, Pusan, Korea 4. Department of Anesthesiology, Pusan National University, 602-061, Pusan, Korea 5. Department of Internal Medicine, College of Medicine, Pusan National University, 602-061, Pusan, Korea 6. Department of Biochemistry, College of Pharmacy, Kyung Seong University, 608-736, Pusan, Korea 7. Department of Food and Nutrition College of Natural Science, Dong-A University, 604-714, Pusan, Korea 8. Department of Chemistry, Jisan Junior College, 609-323, Pusan, Korea |
| Abstract: | Synaptosomal plasma membrane vesicles (SPMV) were isolated from fresh bovine cerebral cortex by several well-known procedures, and the best procedure was selected by enzymatic and morphological standards. The SPMV isolated by the modified procedure of Smith and Loh showed the highest purity. The specific activities of Na, K-ATPase, acetylcholinesterase and 5′-nucleotidase were about 5.6-fold, 2.5-fold and 3.3-fold, respetively, enriched in the plasma membrane fraction as compared to those of the crude homogenate. Electron microscopic examination also showed that the membranes were in vesicular form. |
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