HBsAg健康携带者自然转阴后外周血淋巴细胞SCE频率的变化

①目的 探讨ＨＢｓＡｇ健康携带者自然转阴后外周血淋巴细胞姐妹染色单体互换（ＳＣＥ）频率的变化。②方法 用常规法培养外周血淋巴细胞并用５－溴脱氧尿嘧啶核苷诱导ＳＣＥ，用紫外线照射法制备ＳＣＥ标本，对２０例ＨＢｓＡｇ健康携带者５年自然转阴后外周血淋巴细胞的ＳＣＥ频率进行检测，同时将２０例ＨＢｓＡｇ阳性的健康携带者作为配对组，３５例ＨＢｓＡｇ阴性的健康人作为正常对照组进行ＳＣＥ频率的检测。③结果 上述３

A CHANGE OF FREQUENCY OF SISTER CHROMATID IN PERIPHERAL LYMPHOCYTES OF HEALTHY CARRIERS WITH HBsAg AFTER TRANSFORMED TO HBsAg NEGATIVITY NATURALLY

Objective To investigate the change of frequency of sister chromatid exchange (SCE) in the peripheral lymphocytes of healthy carriers with HBsAg after transformed to HBsAg negativity naturally. Methods The peripheral lymphocytes were cultured according to the routine methods. SCE of the peripheral lymphocytes was induced by BrdU and the SCE slides were prepared using the UPG method. The frequency of SCE of 20 healthy carriers with HBsAg after transformed negative in 5 years were observed. The frequencies of 20 healthy carriers with HBsAg positive as a pairing group and 35 normal control individuals were also observed. Results The frequencies of SCE of the three groups were different(F=3.404,P<0.05). The frequency of SCE of the pairing group was significantly higher than that of the control group (q=3.698, P<0.05), but there was no significant difference in SCE between the transformed negative group and pairing group or control group(q=2.017,1.423, P>0.05). Conclusion The human body may have the ability of repairing the DNA damage caused by the infectin of hepatitis B virus. [