| 红系祖细胞在造血缺陷斑马鱼体内的移植 |
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| 引用本文： | 李志操,黄波,向文碧,孙琮杰,吴西军,周艳华,何志旭,舒莉萍. 红系祖细胞在造血缺陷斑马鱼体内的移植[J]. 中国比较医学杂志, 2019, 0(5): 84-89 |
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| 作者姓名： | 李志操 黄波 向文碧 孙琮杰 吴西军 周艳华 何志旭 舒莉萍 |
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| 作者单位： | 贵州医科大学细胞工程生物医药技术国家地方联合工程实验室组织工程与干细胞实验中心临床医学院儿科学教研室贵州省再生医学重点实验室;中国医学科学院成体干细胞转化研究重点实验室;遵义医科大学附属医院儿科学教研室 |
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| 基金项目： | 黔教研合GZS[2016]02;黔科合J重大字[2015]2003;筑科合同[2017]5-18号;黔科合平台人才[2017]5611;中国医学科学院中央级公益性科研院所基本科研业务费专项资金资助(2017PT31042);中国医学科学院中央级公益性科研院所基本科研业务费专项资金资助(2018PT31048) |
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| 摘 要： | 目的评估红系祖细胞在先天原始造血缺陷cloche-/-突变体斑马鱼体内的移植效果。方法收集绿色荧光标记红系祖细胞的转基因系zTg(gata1:EGFP)斑马鱼胚胎,制备成单细胞悬液,经流式细胞仪分选出携带绿色荧光的gata1^+细胞,利用显微注射技术将gata1^+细胞移植到42hpf的cloche-/-突变体斑马鱼心脏中,采用体视荧光显微镜追踪观察移植后的红系祖细胞在cloche-/-突变体斑马鱼中的表达情况。结果成功分选出携带绿色荧光的gata1^+细胞,并在移植后2 h可观察到携带绿色荧光的gata1^+细胞逐渐增殖扩散且16 h持续有绿色荧光表达。结论红系祖细胞有重建造血的潜力,为进一步研究红系祖细胞移植后的功能鉴定提供实验依据。
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| 关 键 词： | 红系祖细胞 斑马鱼 移植 cloche^-/-突变 |
Transplantation of erythroid progenitor cells into hematopoietic deficient zebrafish |
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| LI Zhicao,HUANG Bo,XIANG Wenbi,SUN Congjie,WU Xijun,ZHOU Yanhua,HE Zhixu,SHU Liping. Transplantation of erythroid progenitor cells into hematopoietic deficient zebrafish[J]. Chinese Journal of Comparative Medicine, 2019, 0(5): 84-89 |
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| Authors: | LI Zhicao HUANG Bo XIANG Wenbi SUN Congjie WU Xijun ZHOU Yanhua HE Zhixu SHU Liping |
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| Affiliation: | (National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique,Center for Tissue Engineering and Stem Cell Research, Department of Pediatrics, Clinical Medical College,Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China;Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004;Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003) |
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| Abstract: | LI Zhicao;HUANG Bo;XIANG Wenbi;SUN Congjie;WU Xijun;ZHOU Yanhua;HE Zhixu;SHU Liping(National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique,Center for Tissue Engineering and Stem Cell Research, Department of Pediatrics, Clinical Medical College,Guizhou Province Key Laboratory for Regenerative Medicine, Guizhou Medical University, Guiyang 550004, China;Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004;Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003) |
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| Keywords: | erythroid progenitor cells zebrafish transplantation cloche^-/- mutant |
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