An experimentally-induced acute hepatic failure model in various strains of mice |
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Authors: | Yasuhiro Mizoguchi MD Hiroshi Kuboi Hiroko Tsutsui Shuichi Seki Kenzo Kobayashi Sukeo Yamamoto Seiji Morisawa Ikuya Yano |
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Institution: | 1. The Third Department of Internal Medicine, Osaka City University Medical School, 1-5-7 Asahi-machi, Abeno-ku, 545, Osaka, Japan 2. Osaka Socio-Medical Center Hospital, Osaka557, Japan 3. First Department of Biochemistry, Osaka City University Medical School, 545, Osaka, Japan 4. Department of Bacteriology, Osaka City University Medical School, 545, Osaka, Japan
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Abstract: | When BALB/cAJcl mice are intravenously injected with heat-killedPropionibacterium acnes (P. acnes) followed by an intravenous injection of lipopolysaccharide (LPS) 7 days later, massive necrosis is induced in the liver tissue
and most of the mice die within 24 hours of LPS injection. Using this experimental model, acute hepatic failure was induced
in various strains of mice and the difference in the response was studied. As a result, as in BALB/cAJcl mice, acut hepatic
failure was also induced in BALB/ cAJcl-nu, AKR/J, C3H/HeNJcl, C57BL/6NJcl and DDy mice. However, as an exception, hepatic
cell necrosis was hardly seen and the survival rate was remarkable high in C3H/HeJ mice, which genetically do not respond
to LPS stimulation. These results indicate that for this experimental induction of acute hepatic failure, macrophages must
be activated by the two-step stimulation ofP. acnes and LPS. |
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Keywords: | acute hepatic failure lipopolysaccharide Propionibacterium ances |
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