HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells |
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| Institution: | 1. Department of Clinical Immunology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Skejby, Denmark;2. National HIV Programme, Ministry of Health, Bissau, Guinea-Bissau;3. Bandim Health Project, Indepth Network, Bissau, Guinea-Bissau;4. Department of Infectious Diseases, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Skejby, Denmark;1. Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;3. Neurosurgery Department, Shiraz University of Medical Sciences, Shiraz, Iran;1. Department of Ophthalmology, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;2. Department of Ophthalmology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;3. Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;1. School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, United States;2. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, United States;3. Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, United States;1. Laboratory of Immunology of Microbial Aggression, Program of Immunology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, 8380453, Chile;2. Laboratory of Biomedicine and Regenerative Medicine, Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, University of Chile, Santiago, 8820808, Chile;1. Medical Sciences Faculty of Minas Gerais, Post-Graduate Program in Health Sciences, Minas Gerais, Brazil;2. Department of Physiology and Biophysics, INCT-Nanobiofar, ICB, Federal University of Minas Gerais, Brazil;3. Department of Biological Sciences, Laboratory of Experimental Pathophysiology, Federal University of Ouro Preto, Minas Gerais, Brazil |
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| Abstract: | Human T-lymphotropic virus (HTLV) affects the human immune system in many ways, most notably by inducing proliferation of infected CD4 + T cells, but several other cell types are also affected. To characterize the effects of HTLV infection, we analysed blood samples from HTLV-infected individuals by flow cytometry. Samples were collected from visitors at the HIV clinic in Bissau, Guinea-Bissau. These samples were tested for HTLV and HIV, and 199 were analysed by flow cytometry using panels for B cells, T-cell maturation and activation, regulatory T cells (Tregs) and monocytes. CD80+ cell proportions were significantly higher in HTLV infected than in HTLV uninfected in all B cell subsets. Among T cells, there was no change in cell distribution between maturation stages, but a higher CD25+ proportion among Tregs (61.1 % vs 36.3 %, p < 0.001) in HTLV infected than in HTLV uninfected. The level of CD49d on individual cells was also higher (MFI 2734.5 vs 1,041, p < 0.001). In HTLV infected individuals, CD8 + T cells had a lower proportion of CTLA-4+ (2.5 % vs 3.5 %, 0.048) and higher PD1+ proportion on the CD45RO + subset (81.6 % vs 77.1 %, p < 0.001). Together, these findings point toward reduced regulation in HTLV + patients, which leads to immune activation. This study corroborates previous findings and offers new insight into the effects of HTLV by providing a broad flowcytometric analysis of immune cells in HTLV + individuals. |
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| Keywords: | HTLV HIV Spectral flow cytometry CD49d CD80 Immune dysregulation |
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