Ex vivo expansion of regulatory T cell and T helper 2 cells using a hematosphere culture |
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| Affiliation: | 1. Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Republic of Korea;2. Department of Convergence Medicine, Pusan National University School of Medicine, Yangsan, Republic of Korea;1. University of Chile, Faculty of Physical and Mathematical Sciences, CeBiB, Chile;2. University of Chile, Faculty of Medicine, ICBM, Immunology Disciplinary Program, Chile;3. University of Chile, Faculty of Medicine, Anatomy and Developmental Biology Disciplinary Program, ICBM, Experimental and Molecular Embryology Laboratory, Chile;1. Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark;2. Aarhus University, Aarhus, Denmark;3. Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus, Denmark;4. Department of Medical Endocrinology, Rigshospitalet, Copenhagen, Denmark;5. Department of Biomedicine, Aarhus University, Aarhus, Denmark;6. Steno Diabetes Center Copenhagen, Gentofte, Denmark;7. Steno Diabetes Center, Sjaelland, Denmark;8. University of Copenhagen, Copenhagen, Denmark;1. Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Medical Faculty, Philipps-University of Marburg, Robert-Koch-Str. 8, 35037, Marburg, Germany;2. Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation, National Institute of Mental Health Intramural Research Program, Bethesda, 20814, Maryland, USA;1. Área Inmunología, DEPBIO/IQB - Facultad de Química/Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay;2. Integrative Parasitology, Center for Infectious Diseases, Heidelberg University, Heidelberg, Germany;1. Department of Veterinary and Animal Science, University of Copenhagen, 1870 Frederiksberg C, Denmark;2. Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark;3. Copenhagen Wound Healing Center, Bispebjerg University Hospital, 2400, Copenhagen NV, Denmark;4. Department of Orthopedic Surgery, Herlev Hospital, 2730, Herlev, Denmark;1. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;2. Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;3. Department of Cell and Molecular Biology, Islamic Azad University, Science and Research Branch. Tehran, Iran;4. Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;5. School of Medicine, Bandar Abbas University of Medical Science, Bandar Abbas, Iran;6. Department of Medical Genetics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Regulatory T cells (T-reg) are important components of immune system required to understand the mechanistic details of cancer immunity and autoimmune diseases. However, reliable and efficient methods of regulatory T cell expansion have not been established yet. Here, we show that a human peripheral blood mononuclear cell (PBMNC) derived blood-born hematosphere (BBHS) culture without cytokine treatment increased T-reg and T helper 2 cell (Th2) populations in the cell suspension. We found that the isolated cells suspended around the hematospheres expressed T helper cell markers. Importantly, the Foxp3+/CD25+ T-reg and IL-4+/CD25+ Th2 cell populations in the cell suspension were greatly expanded during hematosphere culture. Through ELISA analysis of the supernatant, we showed that secretion of Th2-related cytokines such as IL-5, IL-10, and IL-13 also increased. Taken together, hematosphere culture is a good method for ex vivo expansion of T-reg and Th2, which can provide therapeutic benefits for the treatment of immune disorders. |
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| Keywords: | Regulatory T cell T helper 2 cell Monocyte Hematosphere BBHS |
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