Multinucleated giant cell phenotype in response to stimulation |
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| Affiliation: | 1. Área Inmunología, DEPBIO/IQB - Facultad de Química/Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay;2. Integrative Parasitology, Center for Infectious Diseases, Heidelberg University, Heidelberg, Germany;1. Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triângulo Mineiro, Uberaba, MG, 38025-350, Brazil;2. Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA;3. Laboratory of Immunology and Infectious Diseases, Triângulo Mineiro Federal University, Uberaba, Brazil;1. Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland;2. Institute of Tuberculosis and Lung Diseases, Plocka 26, 01-138, Warsaw, Poland;3. The Voivodeship Hospital of Lung Diseases in Jaroszowiec, Kolejowa 1a, 32-312, Jaroszowiec, Poland;4. Masovian Center of Lung Diseases and Tuberculosis Treatment, Narutowicza 80, 05-400, Otwock, Poland;5. Department of Biomedicine, Aarhus University, Høegh-Guldbergs Gade 10, 8000, Aarhus, Denmark;6. Laboratory of Mycobacterium Genetics and Physiology, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland;1. Key Laboratory of Molecular Mechanism and Intervention Research for Plateau Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, 712082, China;2. Department of Emergency, Affiliated Hospital of Xizang Minzu University, Xianyang, Shaanxi, 712082, China;3. School of Basic Medical Sciences, Xizang Minzu University, Xianyang, Shaanxi, 712082, China;4. Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Northwest University, Xi’an, Shaanxi, 710069, China;1. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;2. Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;3. Department of Cell and Molecular Biology, Islamic Azad University, Science and Research Branch. Tehran, Iran;4. Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;5. School of Medicine, Bandar Abbas University of Medical Science, Bandar Abbas, Iran;6. Department of Medical Genetics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Macrophages fuse into multinucleated giant cells (MGC) in many pathological conditions. Despite MGC correlations with granulomas, their functional contribution to inflammation is relatively unknown. An in vitro mouse model of IL-4-induced bone marrow-derived macrophage fusion and microfiltration were used to generate enriched MGC and macrophage populations. Phenotypes were compared in response to well-known inflammatory stimuli, including lipopolysaccharide and crocidolite asbestos. Surface markers were assessed by flow cytometry: CD11b, CD11c, F4/80, and MHC II. Secreted cytokines were assessed by multiplex immunoassay: IFN-γ, IL-1β, IL-6, TNF-α, IL-10, IL-13, and IL-33. Results show that MGC maintained macrophage surface protein expression but lost the ability to produce a cytokine response. This suggests a potentially beneficial role of MGC in isolating the host from a foreign body without contributing to excessive inflammation. This study and future research using other stimulants and environments are important to gaining a fundamental MGC cell biology understanding. This will inform approaches to controlling the foreign body response to particle exposure, medical implants, and many diseases associated with granulomas. |
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| Keywords: | Multinucleated giant cell Macrophage In vitro Mouse Phenotype Surface marker Cytokine |
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