3种戊型肝炎病毒抗原检测血清抗—HEV IgG的比例研究

目的：用3种抗原检测国家戊型肝炎参比血清和急性戊型肝炎病人血清抗－HEV IgG。方法：人工合成戊型肝炎病毒（HEV）ORF2和ORF3多肽抗原（BMU抗原）检测参比血清的灵敏度（90．0％），特异度（100．0％）和符合率（97．5％）均高于杆状病毒表达的HEV重组蛋白空粒子抗原（JP抗原）（分别为70．0％，93．3％和87．5％）及大肠杆菌表达的重组蛋白抗原（HKU抗原）（分别为70．0％，90．0％和85．0％），检测84例急性戊型肝炎病人血清抗－HEV IgG，用BMU抗在检测抗－HEV IgG的阳性率为1000％（84／84），高于JP抗原（83／84，98．8％）和HKU抗原（71．84，84．5％）。结果：不同HEV抗原检测国家参比血清和急性戊型肝炎病人血清抗－HEV IgG的灵敏度和特异性不同，其差异主要与编码该3种抗原的HEV基因片段不同有关，ORF3抗原在检测急性HE病人中具有重要意义。联合应用ORF2和ORF3抗原可提高抗－HEVIgG检出率，结论：抗－HEV IgG ELISA诊断试剂至少应包括ORF2和ORF3 2种抗原。

Compariative Study on Detecting Anti-HEV IgG in Sera Using Three Different Hepatitis E Virus Antigens

Objective The reference panel of anti HEV IgG and sera collected from patients with acute hepatitis E were detected using three different hepatitis E virus(HEV) antigens enzyme linked immune assays (ELISAs).Methods The sensitivity(90 0%) and specificity(100 0%) and coincidence rates(97 5%) of the synthetic HEV peptides(BMU antigen)were higher than those of the two recombinant proteins (JP antigen and HKU antigen) for detection of anti HEV IgG in the reference panel.The anti HEV IgG positive rate of BMU ELISA(100 0%) was higher than those of JP and HKU(98 8% and 84 5%,respectively)when the patients,sera with acute hepatitis E were tested.Results The data showed that different HEV antigens had different sensitivity and specificity for detection of anti HEV in sera.The antigen encoded by HEV LRF3 was important for detecting antibody in acute sera of patients with hepatitis E.Conclusion Both ORF2 and ORF3 antigens should be used for development of anti HEV IgG ELISA kit.