| 丹参及丹参注射液指纹图谱的HPLC-MS研究 |
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| 引用本文： | 张尊建,李茜,王伟,邹巧根,赵陆华.丹参及丹参注射液指纹图谱的HPLC-MS研究[J].医学教育探索,2002(12):1074-1076. |
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| 作者姓名： | 张尊建 李茜 王伟 邹巧根 赵陆华 |
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| 作者单位： | 中国药科大学分析测试中心 江苏南京210009
(张尊建，李茜，王伟，邹巧根)，中国药科大学分析测试中心 江苏南京210009(赵陆华) |
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| 摘 要： | 目的 采用HPLC－UV－MS法对丹参药材、丹参注射液中间体及丹参注射液进行指纹图谱的研究。方法 采用Alltima C18分析柱，甲醇－水－冰醋酸梯度洗脱系统，流速：1mL/min，检测波长：281nm，MS册时记录总离子流（TIC）色谱图。结果 得到分离度较好的丹参药材、中间体及注射液的HPLC－UV及HPLC－MS指纹图谱。结论为丹参药材、中间体及注射液的质量控制提供全面、可靠的依据。
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| 关 键 词： | 丹参 丹参注射液 指纹图谱 HPLC－UV HPLC－MS |
Study on fingerprints of Radix Salviae Miltiorrhizae and its INJECTION by HPLC-MS |
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| ZHANG Zun-jian,LI Qian,WANG Wei,ZOU Qiao-gen,ZHAO Lu-hua.Study on fingerprints of Radix Salviae Miltiorrhizae and its INJECTION by HPLC-MS[J].Researches in Medical Education,2002(12):1074-1076. |
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| Authors: | ZHANG Zun-jian LI Qian WANG Wei ZOU Qiao-gen ZHAO Lu-hua |
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| Abstract: | Object To investigate the chromatographic fingerprints of Radix Salviae Miltiorrhizae (RSM), its intermediate and INJECTION by HPLC-UV-MS. Methods Separation was performed on an Alltima C 18 analytical column with the mobile phase consisting of methanol-water-acetic acid as gradient eluent at the flow rate of 1 mL/min. The UV detection was set at 281 nm and TIC was recorded by an electrospray ionization mass spectrometer in negative ion mode. Results The HPLC-UV and HPLC-MS fingerprints of RSM, its intermediate and INJECTION were obtained with perfect isolation. Conclusion The fingerprints could be used for the control of RSM, its intermediate and INJECTION. |
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| Keywords: | Radix Salviae Miltiorrhizae (RSM) RSM INJECTION fingerprint HPLC-UV HPLC-MS |
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