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| 鸡新城疫病毒口服微球的制备研究 | |
| 引用本文： | 胡巧红,许东航,王松. 鸡新城疫病毒口服微球的制备研究[J]. 中国药房, 2006, 17(10): 740-743 |
| 作者姓名： | 胡巧红 许东航 王松 |
| 作者单位： | 1. 广东药学院,广州市,510006;浙江大学药学院,杭州市,310031 2. 浙江大学医学院附属第二医院,杭州市310009 3. 浙江大学药学院,杭州市,310031 |
| 摘 要： | 目的:研究口服疫苗微球的最佳制备工艺。方法:以鸡新城疫病毒(NDV)作为疫苗模型药物、乳酸-羟基乙酸共聚物(PLGA)为载体材料,采用W/O/W复乳-溶剂挥发法制备NDV-PLGA口服微球。采用正交设计试验,考察活NDV体积、PL GA浓度、初乳搅拌速度、内水相中的保护剂对微球形状、粒径、粘连情况和药物活性的影响,其中以血凝法测定微球中释放药物的效价。结果:NDV口服微球的最佳制备工艺系活NDV体积为200μl、PLGA浓度为4%、初乳搅拌速度为8000r/min、内水相中的保护剂为牛血清白蛋白。结论:本方法为制备口服疫苗微球的最佳制备工艺。 |
| 关 键 词： | 鸡新城疫病毒 口服疫苗微球 血凝法 效价 |
| 文章编号： | 1001-0408（2006）10-0740-04 |
| 收稿时间： | 2006-01-06 |
| 修稿时间： | 2006-03-27 |
Preparation of Oral Vaccine Microspheres for Newcastle Disease Virus |
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| HU Qiaohong,XU Donghang,WANG Song. Preparation of Oral Vaccine Microspheres for Newcastle Disease Virus[J]. China Pharmacy, 2006, 17(10): 740-743 | |
| Authors: | HU Qiaohong XU Donghang WANG Song |
| Affiliation: | Guangdong Pharmaceutical College, Guangzhou 510006, China;The Second Affiliated Hospital of Medical College of Zhejiang University, Hangzhou 310009, China;College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China |
| Abstract: | OBJECTIVE:To study the preparation technique for oral vaccine microspheres.METHODS:Newcastle dis-ease virus(NDV)vaccine was used as model drug,and PLGA as carrying agent to prepare NDV-PLGA oral microspheres by W/O/W emulsifying-solvent evaporation technique.Orthogonal design experiment was carried out to determine the influence of NDV volume,PLGA concentration,stirring speed and protectants at inner aqueous phase on the shape,size,adhesion and activity of microspheres.The valence of drug release in microspheres was measured by hemagglutination.RESULTS:The best condition for preparing NDV microspheres was found to be200μl of NDV,4%PLGA,stirring speed at8000r/min,and BSA as protectant.CONCLUSION:The established technique is the optimal choice for preparation of oral vaccine micro-spheres. |
| Keywords: | Newcastle disease virus Oral vaccine microspheres Hemaggltination Valence |
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