| 人类骨肉瘤细胞的血管形成因子研究 |
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| 引用本文： | WUHua DENGZhongduan 等. 人类骨肉瘤细胞的血管形成因子研究[J]. 中德临床肿瘤学杂志, 2002, 1(2): 94-97. DOI: 10.1007/BF02851743 |
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| 作者姓名： | WUHua DENGZhongduan 等 |
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| 作者单位： | [1]DepartmentofOrthopedics,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030 [2]DepartmentofPathology,SchoolofBasicMedicalSciences,TonjiMedicalColl |
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| 摘 要： | Objective To partially purify the angiogenesis factor of human osteosarcoma(HuOs) and study its biological features. Methods The active peptide with a molecular weight of 8000-10000 Da in the conditioned medium obtained from the cultivation of Hu-Os cells(osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and dialysis.The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells. Results The chromatography fractions 4-6 could significantly promote the proliferation of the endothelial cells.Conclusion The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000-10000 Da.
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| 关 键 词： | 骨肉瘤 血管形成因子 部分纯化 |
| 收稿时间： | 2002-03-12 |
The partial purification of angiogenesis factor of human osteosarcoma |
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| Wu Hua,Deng Zhongduan,Chen Duoen. The partial purification of angiogenesis factor of human osteosarcoma[J]. The Chinese-German Journal of Clinical Oncology, 2002, 1(2): 94-97. DOI: 10.1007/BF02851743 |
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| Authors: | Wu Hua Deng Zhongduan Chen Duoen |
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| Affiliation: | (1) Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China;(2) Department of Pathology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China |
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| Abstract: | Objective To partially purify the angiogenesis factor of human osteosarcoma (HuOs) and study its biological features. Methods The active peptide with a molecular weight of 8 000–10 000 Da in the conditioned medium obtained from the cultivation of Hu-Os cells (osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and dialysis. The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells. Results The chromatography fractions 4–6 could significantly promote the proliferation of the endothelial cells. Conclusion The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8 000–10 000 Da. |
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| Keywords: | osteosarcoma angiogenesis factor |
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