HPLC测定附子对大鼠肝组织中腺苷酸含量及能荷的影响

目的: 采用反相高效液相色谱(HPLC)技术,探讨中药附子对正常大鼠肝组织中三磷酸腺苷(ATP),二磷酸腺苷(ADP)及一磷酸腺苷(AMP)含量及其对能荷(EC)水平的影响。 方法: 将20只Wister大鼠随机分为2组,分别灌胃附子水煎液和生理盐水4周,4周后麻醉处死,取肝组织进行反相高效液相色谱测定。 结果: 附子给药组大鼠较对照组大鼠肝脏中ATP含量明显升高,提高了70.3%,具有极显著性差异( P <0.01),ADP,AMP的含量2组间无明显变化,总腺苷酸库、EC水平附子给药组与对照组比亦显著性升高( P <0.05)。 结论: 热性中药附子可以增加机体能量储备,能显著提高机体肝组织的ATP含量和EC水平,起到影响肝脏能量代谢的作用。

Determination of Contents of Adenine Nucleotides and Energy Charge in Rat Liver Tissue Treated with Aconite Roots by HPLC

Objective: To investigate the influence of traditional Chinese medicine aconite roots on the contents of adenosine triphosphate (ATP),adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in normal rats liver tissue and its impact on the energy charge by RP-HPLC. Method: 20 Wister rats were divided into two groups randomLy and were administered with decoction of aconite roots or saline respectively for four weeks.The contents of adenine nucleotides in rat liver tissues were analysed by RP-HPLC after four weeks. Result: The hepatic ATP in aconite roots treated group increased significantly by 70.3% ( P <0.01),with high statistical significancy compared with that in control group,whereas the content of ADP and AMP showed no obvious differences between these two groups.The total adenylate library and energy charge (EC) levels in aconite roots treated group also increased significantly( P <0.05) compared with that in the control group. Conclusion: Aconite roots,the hot natured Chinese herbal medicine,can boost the body energy reserves,increase the ATP content and EC level in liver tissue,and thus influence the hepatic energy metabolism.