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| SARS冠状病毒BJ01株基因组全长cDNA的构建与鉴定 | |
| 引用本文： | 赵卓,韩剑峰,陈水平,秦成峰,姜涛,于曼,何维明,秦鄂德. SARS冠状病毒BJ01株基因组全长cDNA的构建与鉴定[J]. 军事医学科学院院刊, 2005, 29(1): 1-3 |
| 作者姓名： | 赵卓 韩剑峰 陈水平 秦成峰 姜涛 于曼 何维明 秦鄂德 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西杨凌,712100;军事医学科学院微生物流行病研究所,北京,100071 2. 军事医学科学院微生物流行病研究所,北京,100071 3. 西北农林科技大学动物科技学院,陕西杨凌,712100 |
| 基金项目： | 国家“973”计划项目 (2003CB514119 )，国家自然科学基金项目(30340021) |
| 摘 要： | 目的:构建我国自行分离的SARS冠状病毒BJ01株基因组全长cDNA分子,为阐明SARS病毒致病的分子机制奠定基础。方法:采用分段克隆的方法对病毒全基因组进行分段扩增和克隆,然后将全基因组各cDNA片段分别进行体外连接获得全长cDNA分子,并对其接头序列进行PCR扩增和测序鉴定。结果与结论:获得了SARS病毒BJ01株基因组全长cDNA分子,对其接头处序列的测定结果表明,该全长cDNA分子为SARS病毒BJ01株的特异序列。 |
| 关 键 词： | SARS冠状病毒 cDNA克隆 全长cDNA分子 |
| 文章编号： | 1000-5501(2005)01-0001-03 |
| 修稿时间： | 2004-12-30 |
Assembly and identification of a full-length cDNA of SARS coronavirus BJ01 strain |
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| Zhao Zhuo,HAN Jian-Feng,CHEN Shui-Ping,QIN Cheng-Feng,JIANG Tao,YU Man,HE Wei-ming,QIN E-De. Assembly and identification of a full-length cDNA of SARS coronavirus BJ01 strain[J]. Bulletin of the Academy of Military Medical Sciences, 2005, 29(1): 1-3 | |
| Authors: | Zhao Zhuo HAN Jian-Feng CHEN Shui-Ping QIN Cheng-Feng JIANG Tao YU Man HE Wei-ming QIN E-De |
| Affiliation: | ZHAO Zhuo 1,2,HAN Jian-Feng 2,CHEN Shui-Ping 2,QIN Cheng-Feng 2,JIANG Tao 2,YU Man 2,HE Wei-Ming 1,QIN E-De 2* |
| Abstract: | Objective: To obtain the genomic full-length cDNA of SARS-CoV BJ01 strain isolated in China, and lay the foundation for clarification of the molecular mechanism of pathogenesis. Methods: First,seven cDNA fragments spanning the entire genome of SARS coronavirus were amplified by RT-PCR and cloned into vectors. Then, the subclones were digested with the appropriate restriction enzymes and assembled systematically in vitro.The junctions of cDNA were sequenced. Results and Conclusion: A full-length cDNA of SARS coronavirus was obtained and proved to be consistent with that of SARS-CoV BJ01 strain. |
| Keywords: | SARS-CoV BJ01 strain cDNA clone full-length cDNA |
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