双向电泳分析快速老化痴呆鼠脑蛋白质的异常表达及针刺的影响

目的:从整体上观察快速老化及伴随的痴呆对脑蛋白质组的影响以及针刺对其的调节作用。方法:以8月龄快速老化痴呆鼠SAMP10和同源正常老化鼠SAMR1为材料,分为P10针刺组、P10非穴组、P10对照组和R1对照组。针刺组施以“益气调血,扶本培元”针法,针刺“膻中”“中脘”“气海”“血海”“足三里”;非穴组针刺双胁下非穴固定点。双向电泳展示各组小鼠大脑蛋白图谱。结果:双向电泳显示,伴随着快速老化和痴呆,3个蛋白发生了质的变化,其中一个蛋白为P10所特有,两个为R1所特有;9个蛋白发生了量的变化,其中7个蛋白伴快速老化和痴呆高表达,2个低表达。针刺穴位具有良性调节作用,可明显改善上述某些蛋白的表达异常,减缓衰老;而非穴无特异性。结论:伴快速老化,SAMP10痴呆鼠大脑某些蛋白的表达发生了异常,针刺可改善其中某些蛋白的异常,并具有穴位特异性。

Analysis on the Aberrant Expression of Cerebral Proteins and the Therapeutic Effect of Acupuncture in SAMP10 by Bi-dimensional Electrophoresis

Objective: To explore the effect of accelerated aging and dementia on cerebral proteome and the regulative effect of acupuncture interference.Methods: Forty eight-month-old senescence-accelerated mice prone10(SAMP10) and their homologue,normal aging mice(SAMR1) of the same age were randomly and evenly assigned to P10-acupuncture,P10-sham-acupuncture,P10-control and R1-control groups with 10 cases in each group.P10-acupuncture group was treated by acupuncture of "Shanzhong"(CV 17),"Zhongwan"(CV 12),"Qihai"(CV 6),"Xuehai"(SP 10) and "Zusanli"(ST 36),once daily and continuously for 15 days except the 7~(th) day.The sham-acupuncture group was treated by acupuncture of non-acupoint at the hypochondrium.The acupuncture needle was twirled rapidly with reinforcing method about(30 s) every time for each acupoint.At the end of the experiments,the mice under deep anesthesia were killed for sampling the brain tissue.After a series of process of the brain tissue,the expressive profiles of cerebral proteins were displayed by applying bi-dimensional electrophoresis technique.Results: With the accelerated senescence and dementia,the expression of 1 protein spot((100 kD/pI 5.5)) appeared and 2 proteins disappeared simultaneously in SAMP10 of P10-acupuncture,P10-sham-acupuncture and P10-control groups,but appeared in SAMR1.In addition,in SAMP10 group,6 proteins displayed abnormal expression including 2 proteins being down-regulated and 4 proteins up-regulated in the expression level which were reversed in P10-acupuncture group,suggesting acupuncture may alleviate the effect of aging and dementia.In P10-sham-acupuncture group,of the 4 proteins being up-regulated in the expression in SAMP10 group, 2 proteins showed up-regulation and the other 2 down-regulation.Conclusion: Some proteins of the cerebrum in SAMP10 show aberrant expression with the accelerated senescence,which can be improved by acupuncture.