不同细胞因子协同活化的T细胞抗肿瘤活性的研究

目的:探讨不同细胞因子协同活化的T细胞抗肿瘤的活性。方法:采用CD3AK法,CD3/CD28共刺激法,CIK法,并作细胞扩增能力检测。测定刺激细胞对肿瘤的杀伤活性,及用流式细胞仪检测共刺激后的T细胞表型变化。结果:抗CD3单克隆抗体可通过CD3成分启动T细胞,使淋巴细胞表型发生变化,联系细胞因子诱导的T淋巴细胞能有效提高T细胞对鼻咽癌细胞的杀伤活性,CIK方法诱导的T淋巴细胞在体外能保持良好的扩增活性。结论:不同细胞因子协同活化的T细胞抗肿瘤活性不同,CD28联合CD3诱导的T细胞抗肿瘤活性较高,CIK法效果最好。

The study of T lymphocytes activated by different cytokind unit and its anti -tumor activation

Objective:To explore T lymphocytes activated by different cytokind unit and its anti-tumor activation.Methods:CD3AK,CD3/CD28,CIK were used and cellular amplification was detected.The cellular phenotype was detected by flow cytometer.Results:Anti-CD3mAb activated T lymphocytes by CD3,and changed phenotype.The T lymphocytes that united cytokines induced were able to increase killing activity of nasopharyngeal carcinoma cells.The T lymphocytes that CIK methods induced have better amplification in vitro.Conclusion:T lymphocytes activated by different cytokine unite had different anti-tumor activation.