| 昆明鼠自体卵丘细胞与早期胚胎序贯共培养对胚胎体外发育潜能的影响 |
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| 引用本文： | 李娟,王磊光,魏斌,王洪岩,邱毅,张丽红,张琦,张梅心,江平,于小洁.昆明鼠自体卵丘细胞与早期胚胎序贯共培养对胚胎体外发育潜能的影响[J].中国计划生育学杂志,2011,19(2):77-80. |
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| 作者姓名： | 李娟 王磊光 魏斌 王洪岩 邱毅 张丽红 张琦 张梅心 江平 于小洁 |
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| 作者单位： | 山东省计划生育科学技术研究所,山东省优生重点实验室,济南,250002 |
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| 基金项目： | 山东省人口和计划生育委员会科研立项(200910); 山东省科技攻关计划(031050134) |
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| 摘 要： | 目的:评价昆明鼠胚胎与自体卵丘细胞序贯共培养对提高早期胚胎体外发育潜能的影响。方法:分两组进行实验,序贯共培养组使用分离培养的卵丘细胞与序贯培养液构建培养体系,与昆明鼠早期胚胎进行共培养;序贯培养组使用序贯培养液对早期胚胎进行序贯培养,观察两组胚胎各阶段囊胚形成率,检测囊胚总细胞数、囊胚细胞凋亡数,囊胚进一步培养获得的干细胞系数。结果:序贯共培养3d后总的囊胚形成率为68.13%,序贯培养组囊胚形成率为45.31%(P<0.05);囊胚总细胞数分别为76.52和38.57(P<0.05),细胞凋亡率分别为7.44%和16.28%(P<0.05)。将囊胚进一步培养,共培养组干细胞建系率为12.50%,序贯培养组未能建系。结论:昆明鼠胚胎与自体卵丘细胞序贯共培养与序贯培养比较,前者能更好地模拟体内环境,提高早期胚胎体外发育潜能。
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| 关 键 词： | 序贯共培养 卵丘细胞 胚胎 |
in vitro development of mouse pronuclear embryos to blastocysts in sequential media with co-culture of autologous cumulus cells |
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| Li Juan,Wang Leiguang,Wei Bin,Wang Hongyan,Qiu Yi,Zhang Lihong,Zhang Qi,Zhang Meixin,Jiang Ping,Yu Xiaojie.in vitro development of mouse pronuclear embryos to blastocysts in sequential media with co-culture of autologous cumulus cells[J].Chinese Journal of Family Planning,2011,19(2):77-80. |
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| Authors: | Li Juan Wang Leiguang Wei Bin Wang Hongyan Qiu Yi Zhang Lihong Zhang Qi Zhang Meixin Jiang Ping Yu Xiaojie |
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| Institution: | Li Juan,Wang Leiguang,Wei Bin,Wang Hongyan,Qiu Yi,Zhang Lihong,Zhang Qi,Zhang Meixin,Jiang Ping,Yu Xiaojie Key Laboratory for Improving Birth Outcome Technique,Shandong Provincial Institute of Science and Technology for Family Planning,Jinan 250002 |
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| Abstract: | Objective:To explore the effect of co-culture of autologous cumulus cells in sequential media on preimplantation embryo development. Methods:Pronuclear embryos of Kunming mice were cultured to blastocysts in sequential media,with and without mouse primary cultures of cumulus cells. Blastocyst formation rates,cell numbers and apoptotic rates of in vitro developing embryos in the different culture systems were compared to each other. Results:The blastocyst formation rate for co-culture systems was significant... |
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| Keywords: | In sequential media with co-culture Cumulus cell Embryo |
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