增殖细胞核抗原表达与鼻咽癌放射敏感性关系的研究

[目的]探讨增殖细胞核抗原(PCNA)的表达与鼻咽癌(NPC)放射敏感性的关系.[方法]采用PCNA抗体进行ABC法免疫组化染色.检测108例患者初行放疗前鼻咽癌组织中PCNA表达情况,显微镜下计数阳性细胞,计算增殖指数(PI),并将所有病例按照PI值的高低以25%为界划分两组;高PCNA表达者(HPI)>25%,低P...

Study of the Detection of Proliferating Cell Nuclear Antigen for Predicting the Radiosensitivity of Nasopharyngeal Carcinoma

[Objective]To explore the relationship between the expression of proliferating cell nuclear antigen （PCNA） and radiosensitivity of nasopharyngeal carcinoma（NPC）. [Methods] PCNA expression in the tissues of 108 NPC patients was examined by PCNA antibody immunohistochemical staining by ABC assay before radiotherapy. The positive cells were counted under microscope. Proliferation index（PI） was also caleulated. According to PI, all patients were divided into high PCNA expression group（HPI with PI〉 25%） and low PCNA expression group（LPl, with PI≤25%）. CT or MRI and pharyngorhinoscopy were performed respectively while the doses of radiotherapy were DT36GY and DT70GY. The tumor regression was observed. [Results] All 108 NPC tissue slices expressed PCNA. Among them, 52 cases were HPI and 56 cases were LPl. The tumor regression of HPI was higher than that of LPI. After radiotherapy, the residual of primary tumor of HPI was lower than that of LPI. There was statistical significance between them. [Conclusion]The expression of PCNA is significantly related with radiosensitivity of NPC, so it is an important index for the radiosensitivity of NPC.