N-乙酰半胱氨酸对大鼠肝纤维化肝中氧化及抗氧化的影响

目的]探讨N-乙酰半胱氨酸(NAC)对大鼠肝纤维化肝中氧化及抗氧化体系的影响.方法]用猪血清大鼠腹腔内注射复制免疫损伤性肝纤维化模型,以NAC为干预组,于第12周处死动物测定肝中过氧化氢酶(CAT)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、和维生素E(Vit E)、同时用Masson's三色...

Effect of N-acetylcysteine on Oxidation and Antioxidation in the Liver of Rat Liver Fibrosis

Objective] To investigate the effect of N-acetylcysteine（NAC） on the oxidation and antioxldation system in the liver of rat hepatic fibrosis. Methods] Immunolesion liver fibrosis models were copied by u sing pig serum injected into rat peritoneal cavity. NAC group was intervention group. Animals were killed at 12 weeks. Hydrogen peroxidase（CAT）, malondialdehyde（MDA）, superoxide dismutase （SOD） , glutathione （GSH） and vitamine E（Vit E） in the liver were measured. Masson＇s dying was used to analyze the extent of hepatic fibrosis in each group, and their relationship was analyzed. Results] SOD, GSH, CAT and Vit E in the liver of NAC group were significantly higher than those of model group, but MDA in NAC group was obviously lower than that in model group. The correlation analysis showed that the extent of liver fibrosis was significantly positively related with MDA（ P〈0.01）, and negatively related with SOD, GSH, Vit E and CAT （ P〈0.01, P〈0.01, P〈0.01, P〈0.01, respectively）.Conelusion]The increase of hepatic collagen ex pression may be correlated with lipid peroxidation. NAC can effectively decrease lipid peroxidation, and restore the balance of oxidation and antioxidation system, and alleviate hepatic fibrosis.