子痫前期发病中CD4＋CD25＋Treg细胞与Foxp3的检测及意义

[目的]研究CD4＋ CD25＋调节性T细胞（CD4＋ CD25＋Treg细胞）和Foxp3基因表达与子痫前期发病的相关性.[方法]选择住院确诊的妊娠期高血压孕妇12例（A组）、轻度子痫前期（MPE）孕妇14例（B组）、重度子痫前期（SPE）孕妇15例（C组）,30例正常妊娠晚期孕妇为对照组（D组）,应用流式细胞术检测并比较四组外周血中CD4＋ CD25＋ Treg和 Foxp3的表达水平.[结果]A组、B组和C组孕妇外周血CD4＋ CD25＋ Treg 和Foxp3表达水平均显著低于对照组（P〈0.05） C组外周血CD4＋ CD25＋ Treg和Foxp3表达水平显著低于A组和B组（P〈0.05）.[结论]子痫前期患者体内存在CD4＋ CD25＋ Treg细胞的异常,CD4＋ CD25＋Treg细胞的数量的减少和Foxp3表达的降低使CD4＋ CD25＋Treg细胞免疫抑制功能减弱,从而使母胎免疫耐受失衡,导致子痫前期的发生.

Detection of CD4+ CD25+ Treg Cells and Foxp3 in Pathogenesis of Preeclampsia

[Objeetive] To investigate the relationship between CD4＋ CD25 ＋ Treg cells, Foxp3 gene expression and the pathogenesis of preeclampsIa. [Methods] Twelve pregnant women with gestational hypertension（group A）, 14 mild preeclampsia（MPE） pregnant women（group B）, 15 severe preeclampsia（SPE） pregnant women（group C） and 30 normal pregnant women（group D） were chosen in hospital Flow cytometry was used to detect and compare CD4＋ CD25＋ Treg cells and expression level of Foxp3 in peripheral blood among 4 groups. [Results] The CD4＋ CD25＋ Treg ceils and Foxp3 expression level in peripheral blood in group A, B and C were significantly lower than those in control group（ P〈0.05）. The CD4＋ CD25＋ Treg cells and Foxp3 expression level in peripheral blood in group C were significantly lower than those in group A and B（ P〈0. 05）. [Conclusion] Preeclampsia patients have abnormal CD4＋ CD25＋ Treg cells. The decrease of CD4＋ CD25＋Treg cells and Foxp3 expression attenuates the immune suppression function of 4＋ CD25＋ Treg cells, thus maternal fetal immune tolerance becomes imbalance which results in the occurrence of preeelampsia.